Cell-free gene expression: an expanded repertoire of applications
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …
It has been used for more than 50 years across the life sciences as a foundational research …
Cell-free gene expression
D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …
specific applications in protein synthesis and labelling for structural biology and proteomics …
Exploring the potential of cell-free protein synthesis for extending the abilities of biological systems
Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …
A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
Glycosylation plays important roles in cellular function and endows protein therapeutics with
beneficial properties. However, constructing biosynthetic pathways to study and engineer …
beneficial properties. However, constructing biosynthetic pathways to study and engineer …
Escherichia coli Mimetic Gold Nanorod-Mediated Photo- and Immunotherapy for Treating Cancer and Its Metastasis
J Hwang, EK An, SJ Kim, W Zhang, JO ** - ACS nano, 2022 - ACS Publications
Most cancer-related deaths are due to metastasis or recurrence. Therefore, the ultimate goal
of cancer therapy will be to treat metastatic and recurrent cancers. Combination therapy for …
of cancer therapy will be to treat metastatic and recurrent cancers. Combination therapy for …
Current technologies to endotoxin detection and removal for biopharmaceutical purification
M Schneier, S Razdan, AM Miller… - Biotechnology and …, 2020 - Wiley Online Library
Endotoxins are the major contributors to the pyrogenic response caused by contaminated
pharmaceutical products, formulation ingredients, and medical devices. Recombinant …
pharmaceutical products, formulation ingredients, and medical devices. Recombinant …
Methodologies for preparation of prokaryotic extracts for cell-free expression systems
Cell-free systems that mimic essential cell functions, such as gene expression, have
dramatically expanded in recent years, both in terms of applications and widespread …
dramatically expanded in recent years, both in terms of applications and widespread …
Cell free bacteriophage synthesis from engineered strains improves yield
Phage therapy to treat life-threatening drug-resistant infections has been hampered by
technical challenges in phage production. Cell-free bacteriophage synthesis (CFBS) can …
technical challenges in phage production. Cell-free bacteriophage synthesis (CFBS) can …
Non-canonical amino acid labeling in proteomics and biotechnology
Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique
bioorthogonal chemical groups during de novo synthesis by taking advantage of both …
bioorthogonal chemical groups during de novo synthesis by taking advantage of both …
[HTML][HTML] Methods to reduce variability in E. Coli-based cell-free protein expression experiments
Cell-free protein synthesis (CFPS) is an established biotechnology tool that has shown great
utility in many applications such as prototy** proteins, building genetic circuits, designing …
utility in many applications such as prototy** proteins, building genetic circuits, designing …