Cross-linking mass spectrometry for investigating protein conformations and protein–protein interactions─ a method for all seasons
Mass spectrometry (MS) has become one of the key technologies of structural biology. In this
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …
Mass spectrometry-based protein footprinting for higher-order structure analysis: fundamentals and applications
Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …
functions. Understanding the complexities of protein higher-order structures and dynamics …
Liquid–liquid phase separation by intrinsically disordered protein regions of viruses: Roles in viral life cycle and control of virus–host interactions
Intrinsically disordered proteins (IDPs) are unable to adopt a unique 3D structure under
physiological conditions and thus exist as highly dynamic conformational ensembles. IDPs …
physiological conditions and thus exist as highly dynamic conformational ensembles. IDPs …
Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1
G-protein-coupled receptor (GPCR) kinases (GRKs) selectively phosphorylate activated
GPCRs, thereby priming them for desensitization. Although it is unclear how GRKs …
GPCRs, thereby priming them for desensitization. Although it is unclear how GRKs …
[HTML][HTML] The PfRCR complex bridges malaria parasite and erythrocyte during invasion
The symptoms of malaria occur during the blood stage of infection, when parasites invade
and replicate within human erythrocytes. The PfPCRCR complex, containing PfRH5 (refs.,) …
and replicate within human erythrocytes. The PfPCRCR complex, containing PfRH5 (refs.,) …
Integration of mass spectrometry data for structural biology
Mass spectrometry (MS) is increasingly being used to probe the structure and dynamics of
proteins and the complexes they form with other macromolecules. There are now several …
proteins and the complexes they form with other macromolecules. There are now several …
Cross-linking mass spectrometry discovers, evaluates, and corroborates structures and protein–protein interactions in the human cell
Significant recent advances in structural biology, particularly in the field of cryoelectron
microscopy, have dramatically expanded our ability to create structural models of proteins …
microscopy, have dramatically expanded our ability to create structural models of proteins …
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
Cleavable Cross-Linkers and Mass Spectrometry for the Ultimate Task of Profiling Protein–Protein Interaction Networks in Vivo
M Matzinger, K Mechtler - Journal of proteome research, 2020 - ACS Publications
Cross-linking mass spectrometry (XL-MS) has matured into a potent tool to identify protein–
protein interactions or to uncover protein structures in living cells, tissues, or organelles. The …
protein interactions or to uncover protein structures in living cells, tissues, or organelles. The …
[HTML][HTML] Integrative modelling of biomolecular complexes
PI Koukos, A Bonvin - Journal of molecular biology, 2020 - Elsevier
In recent years, the use of integrative, information-driven computational approaches for
modeling the structure of biomolecules has been increasing in popularity. These are now …
modeling the structure of biomolecules has been increasing in popularity. These are now …