Cereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …

CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Ensuring food security for an ever-growing global population while adapting to climate
change is the main challenge for agriculture in the 21st century. Although new technologies …

CRISPR/Cas enables a targeted modification of DNA sequences. Despite their ease and
efficient use, one limitation is the potential occurrence of associated off-target effects. This …

Genome-editing technologies offer unprecedented opportunities for crop improvement with
superior precision and speed. This review presents an analysis of the current state of …

After being domesticated from teosinte, cultivated maize (Zea mays ssp. mays) spread
worldwide and now is one of the most important staple crops. Due to its tremendous …

The discovery of CRISPR: Cas9 and its application as a powerful gene-editing tool has
transformed the world of basic and applied science, especially the molecular biology dome …

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology
allows the modification of DNA sequences in vivo at the location of interest. Although …

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR
associated protein 9) is a powerful targeted mutagenesis tool that has been implemented in …

Maize (Zea mays L.) is an important crop that has been widely studied for its agronomic and
industrial applications and is one of the main classical model organisms for genetic …