Identification and confirmation of bioactive small‐molecule targets is a crucial, often decisive
step both in academic and pharmaceutical research. Through the development and …

Cellular responses to environmental cues involve the mobilization of GTPases, protein
kinases and phosphoprotein phosphatases. The spatial organization of these signalling …

The medical and pharmaceutical communities are facing a dire need for new druggable
targets, while, paradoxically, the targets of some drugs that are in clinical use or …

Second messenger responses rely on where and when the enzymes that propagate these
signals become active. Spatial and temporal organization of certain signaling enzymes is …

Preclinical stages in the drug discovery process require a multitude of biochemical and
genetic assays in order to characterize the effects of drug candidates on cellular systems …

Stable isotope labeling is at present one of the most powerful methods in quantitative
proteomics. Stable isotope labeling has been performed at both the protein as well as the …

The second messenger cyclic adenosine monophosphate (cAMP), which is produced by
adenylyl cyclases following stimulation of G-protein-coupled receptors, exerts its effect …

The β‐adrenergic receptor/cyclic AMP/protein kinase A (PKA) signalling pathway regulates
heart rate and contractility. Here, we identified a supramolecular complex consisting of the …

A-kinase anchoring proteins (AKAPs) tether the cAMP-dependent protein kinase (PKA) to
intracellular sites where they preferentially phosphorylate target substrates. Most AKAPs …

Phosphodiesterases (PDEs) are a superfamily of enzymes that hydrolyze cyclic nucleotides.
While the 11 PDE subfamilies share common features, key differences confer signaling …