Understanding 3D genome organization by multidisciplinary methods

I Jerkovic, G Cavalli - Nature Reviews Molecular Cell Biology, 2021 - nature.com
Understanding how chromatin is folded in the nucleus is fundamental to understanding its
function. Although 3D genome organization has been historically difficult to study owing to a …

Organization and function of the 3D genome

B Bonev, G Cavalli - Nature Reviews Genetics, 2016 - nature.com
Understanding how chromatin is organized within the nucleus and how this 3D architecture
influences gene regulation, cell fate decisions and evolution are major questions in cell …

Cohesin and CTCF control the dynamics of chromosome folding

P Mach, PI Kos, Y Zhan, J Cramard, S Gaudin… - Nature …, 2022 - nature.com
In mammals, interactions between sequences within topologically associating domains
enable control of gene expression across large genomic distances. Yet it is unknown how …

Targeted degradation of CTCF decouples local insulation of chromosome domains from genomic compartmentalization

EP Nora, A Goloborodko, AL Valton, JH Gibcus… - Cell, 2017 - cell.com
The molecular mechanisms underlying folding of mammalian chromosomes remain poorly
understood. The transcription factor CTCF is a candidate regulator of chromosomal …

Single-cell chromatin accessibility reveals principles of regulatory variation

JD Buenrostro, B Wu, UM Litzenburger, D Ruff… - Nature, 2015 - nature.com
Cell-to-cell variation is a universal feature of life that affects a wide range of biological
phenomena, from developmental plasticity, to tumour heterogeneity. Although recent …

[HTML][HTML] Formation of chromosomal domains by loop extrusion

G Fudenberg, M Imakaev, C Lu, A Goloborodko… - Cell reports, 2016 - cell.com
Topologically associating domains (TADs) are fundamental structural and functional
building blocks of human interphase chromosomes, yet the mechanisms of TAD formation …

Nonlinear control of transcription through enhancer–promoter interactions

J Zuin, G Roth, Y Zhan, J Cramard, J Redolfi… - Nature, 2022 - nature.com
Chromosome structure in mammals is thought to regulate transcription by modulating three-
dimensional interactions between enhancers and promoters, notably through CTCF …

The spatial organization of transcriptional control

A Hafner, A Boettiger - Nature Reviews Genetics, 2023 - nature.com
In animals, the sequences for controlling gene expression do not concentrate just at the
transcription start site of genes, but are frequently thousands to millions of base pairs distal …

Ever-changing landscapes: transcriptional enhancers in development and evolution

HK Long, SL Prescott, J Wysocka - Cell, 2016 - cell.com
A class of cis-regulatory elements, called enhancers, play a central role in orchestrating
spatiotemporally precise gene-expression programs during development. Consequently …

Gene regulation in time and space during X-chromosome inactivation

A Loda, S Collombet, E Heard - Nature Reviews Molecular Cell Biology, 2022 - nature.com
X-chromosome inactivation (XCI) is the epigenetic mechanism that ensures X-linked dosage
compensation between cells of females (XX karyotype) and males (XY). XCI is essential for …