Cell-free gene expression: methods and applications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …
molecules and processes outside of living intact cells. The foundational principle is that …
Enhancing cyclotide bioproduction: harnessing biological synthesis methods and various expression systems for large-scale manufacturing
Peptide-based medications hold immense potential in addressing a wide range of human
disorders and discomforts. However, their widespread utilization encounters two major …
disorders and discomforts. However, their widespread utilization encounters two major …
Beyond In Vivo, Pharmaceutical Molecule Production in Cell-Free Systems and the Use of Noncanonical Amino Acids Therein
MG Casteleijn, U Abendroth, A Zemella… - Chemical …, 2025 - ACS Publications
Throughout history, we have looked to nature to discover and copy pharmaceutical solutions
to prevent and heal diseases. Due to the advances in metabolic engineering and the …
to prevent and heal diseases. Due to the advances in metabolic engineering and the …
In Vitro Reconstitution and Analysis of SARS-CoV-2/Host Protein–Protein Interactions
The emergence of viral threats such as Ebola, ZIKA, and severe acute respiratory syndrome
coronavirus-2 (SARS-CoV-2) requires a rapid and efficient approach for elucidating …
coronavirus-2 (SARS-CoV-2) requires a rapid and efficient approach for elucidating …
Venom biotechnology: casting light on nature's deadliest weapons using synthetic biology
Venoms are complex chemical arsenals that have evolved independently many times in the
animal kingdom. Venoms have attracted the interest of researchers because they are an …
animal kingdom. Venoms have attracted the interest of researchers because they are an …
[HTML][HTML] Venom Ex Machina? Exploring the Potential of Cell-Free Protein Production for Venom Biodiscovery
Venoms are a complex cocktail of potent biomolecules and are present in many animal
lineages. Owed to their translational potential in biomedicine, agriculture and industrial …
lineages. Owed to their translational potential in biomedicine, agriculture and industrial …
Advanced applications of Nanodiscs-based platforms for antibodies discovery
Due to their fundamental biological importance, membrane proteins (MPs) are attractive
targets for drug discovery, with cell surface receptors, transporters, ion channels, and …
targets for drug discovery, with cell surface receptors, transporters, ion channels, and …
A cell-free expression pipeline for the generation and functional characterization of nanobodies
L Haueis, M Stech, S Kubick - Frontiers in bioengineering and …, 2022 - frontiersin.org
Cell-free systems are well-established platforms for the rapid synthesis, screening,
engineering and modification of all kinds of recombinant proteins ranging from membrane …
engineering and modification of all kinds of recombinant proteins ranging from membrane …
A roadmap to the enzymes from spider venom: Biochemical ecology, molecular diversity, and value for the bioeconomy
Spiders are ancient and highly successful predators, which use venom for both predation
and defense. Their venoms are complex mixtures of potent biological molecules, emerging …
and defense. Their venoms are complex mixtures of potent biological molecules, emerging …
[HTML][HTML] Cell-Free Systems: Ideal Platforms for Accelerating the Discovery and Production of Peptide-Based Antibiotics
Peptide-based antibiotics (PBAs), including antimicrobial peptides (AMPs) and their
synthetic mimics, have received significant interest due to their diverse and unique …
synthetic mimics, have received significant interest due to their diverse and unique …