In September 1998 the workshop for “Conditional Genome Alterations” in Cold Spring
Harbor had a simple but important take-home message:“Cre works”(Rossant and McMahon …

Site-specific recombinase systems (Cre-loxP, Flp-FRT, and φC31-att) are transforming both
forward and reverse genetics in mice. By enabling high-fidelity DNA modifications to be …

The mammalian nervous system executes complex behaviors controlled by specialized,
precisely positioned, and interacting cell types. Here, we used RNA sequencing of half a …

How tissues generate and maintain the correct number of cells is a fundamental problem in
biology. In principle, tissue turnover can occur by the differentiation of stem cells, as is well …

The location and lineage of cells that give rise to endocrine islets during embryogenesis has
not been established nor has the origin or identity of adult islet stem cells. We have …

Neural crest cells are multipotential stem cells that contribute extensively to vertebrate
development and give rise to various cell and tissue types. Determination of the fate of …

In recent years, the Cre integrase from bacteriophage P1 has become an essential tool for
conditional gene activation and/or inactivation in mouse. In an earlier report, we described a …

Proper adaptation to environmental perturbations is essential for tissue homeostasis. In the
intestine, diverse environmental cues can be sensed by immune cells, which must balance …

Neural control of the function of visceral organs is essential for homeostasis and health.
Intestinal peristalsis is critical for digestive physiology and host defence, and is often …

The purification, renewal and differentiation of native cardiac progenitors would form a
mechanistic underpinning for unravelling steps for cardiac cell lineage formation, and their …