Cryo-electron tomograms capture a wealth of structural information on the molecular
constituents of cells and tissues. We present DeePiCt (deep picker in context), an open …

During cell division, kinetochore microtubules (KMTs) provide a physical linkage between
the chromosomes and the rest of the spindle. KMTs in mammalian cells are organized into …

The mitotic spindle is a complex three-dimensional (3D) apparatus that functions to ensure
the faithful segregation of chromosomes during cell division. Our current understanding of …

In oocytes of many organisms, meiotic spindles form in the absence of centrosomes [1–5].
Such female meiotic spindles have a pointed appearance in metaphase with microtubules …

Coordination between cell differentiation and proliferation during development requires the
balance between asymmetric and symmetric modes of cell division. However, the cellular …

Skeletal muscle is responsible for voluntary force generation across animals, and muscle
architecture largely determines the parameters of mechanical output. The ability to analyze …

Most female meiotic spindles undergo striking morphological changes while transitioning
from metaphase to anaphase. The ultra-structure of meiotic spindles, and how changes to …

Chromosome segregation during male meiosis is tailored to rapidly generate multitudes of
sperm. Little is known about mechanisms that efficiently partition chromosomes to produce …

Asymmetric partitioning is an essential component of many developmental processes. As
spermatogenesis concludes, sperm are streamlined by discarding unnecessary cellular …

3D segmentation of cytoskeletal filaments and organelles is crucial for studying these
structures in cellular (cryo-) electron microscopy (EM) and tomography (ET). Manual …