In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

The brain is arguably the most complex organ. The branched and extended morphology of
nerve cells, their subcellular complexity, the multiplicity of brain cell types as well as their …

Capturing mitochondria's intricate and dynamic structure poses a daunting challenge for
optical nanoscopy. Different labeling strategies have been demonstrated for live-cell …

Abstract Three-dimensional (3D) reconstruction of living brain tissue down to an individual
synapse level would create opportunities for decoding the dynamics and structure–function …

Mitochondria are highly dynamic organelles that exhibit a complex inner architecture. They
exhibit a smooth outer membrane and a highly convoluted inner membrane that forms …

Bright monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as
protein tags for multicolor microscopy and in vivo imaging. Here we apply rational design to …

A common goal of fluorescence microscopy is to collect data on specific biological events.
Yet, the event-specific content that can be collected from a sample is limited, especially for …

Liver cell types derived from induced pluripotent stem cells (iPSCs) share the potential to
investigate development, toxicity, as well as genetic and infectious disease in ways currently …

Mitochondria are essential for eukaryotic life. These double-membrane organelles often
form highly dynamic tubular networks interacting with many cellular structures. Their highly …

Investigating the interplay of cellular proteins with optical microscopy requires multitarget
labeling. Spectral multiplexing using high-affinity or covalent labels is limited in the number …