Synthetic biology: bottom-up assembly of molecular systems
The bottom-up assembly of biological and chemical components opens exciting
opportunities to engineer artificial vesicular systems for applications with previously unmet …
opportunities to engineer artificial vesicular systems for applications with previously unmet …
Protein engineering design from directed evolution to de novo synthesis
With the development of enzyme engineering technology, green enzyme catalysis is widely
poised to replace traditional chemical catalysis enabling high enantioselectivity and yielding …
poised to replace traditional chemical catalysis enabling high enantioselectivity and yielding …
Baeyer–Villiger monooxygenases: tunable oxidative biocatalysts
Pollution, accidents, and misinformation have earned the pharmaceutical and chemical
industry a poor public reputation, despite their undisputable importance to society …
industry a poor public reputation, despite their undisputable importance to society …
Hot spots for the protein engineering of Baeyer-Villiger monooxygenases
K Balke, A Beier, UT Bornscheuer - Biotechnology advances, 2018 - Elsevier
Abstract Baeyer-Villiger monooxygenases (BVMOs) are versatile biocatalysts for the
conversion of ketones to lactones or esters while also being able to efficiently oxidize …
conversion of ketones to lactones or esters while also being able to efficiently oxidize …
Shifting redox reaction equilibria on demand using an orthogonal redox cofactor
Nature's two redox cofactors, nicotinamide adenine dinucleotide (NAD+) and nicotinamide
adenine dinucleotide phosphate (NADP+), are held at different reduction potentials, driving …
adenine dinucleotide phosphate (NADP+), are held at different reduction potentials, driving …
Protein engineering for nicotinamide coenzyme specificity in oxidoreductases: attempts and challenges
AM Chánique, LP Parra - Frontiers in microbiology, 2018 - frontiersin.org
Oxidoreductases are ubiquitous enzymes that catalyze an extensive range of chemical
reactions with great specificity, efficiency, and selectivity. Most oxidoreductases are …
reactions with great specificity, efficiency, and selectivity. Most oxidoreductases are …
Mutations Increasing Cofactor Affinity, Improve Stability and Activity of a Baeyer–Villiger Monooxygenase
HR Mansouri, O Gracia Carmona, J Jodlbauer… - ACS …, 2022 - ACS Publications
The typically low thermodynamic and kinetic stability of enzymes is a bottleneck for their
application in industrial synthesis. Baeyer–Villiger monooxygenases, which oxidize ketones …
application in industrial synthesis. Baeyer–Villiger monooxygenases, which oxidize ketones …
Switching cofactor dependence of 7β-hydroxysteroid dehydrogenase for cost-effective production of ursodeoxycholic acid
ZN You, Q Chen, SC Shi, MM Zheng, J Pan… - Acs …, 2018 - ACS Publications
Dehydrogenases are widely employed as biocatalysts for the production of optically pure
chemicals under mild conditions. Most dehydrogenases are nicotinamide cofactor (NADPH …
chemicals under mild conditions. Most dehydrogenases are nicotinamide cofactor (NADPH …
Design and engineering of whole-cell biocatalytic cascades for the valorization of fatty acids
JW Song, JH Seo, DK Oh, UT Bornscheuer… - Catalysis Science & …, 2020 - pubs.rsc.org
One of the key factors to construct a productive whole-cell biocatalytic cascade may include
the design of the biotransformation pathways, discovery and engineering of the cascade …
the design of the biotransformation pathways, discovery and engineering of the cascade …
Engineering a Formate Dehydrogenase for NADPH Regeneration
W Ma, Q Geng, C Chen, YC Zheng, HL Yu… - Chembiochem, 2023 - Wiley Online Library
Nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide
phosphate (NADPH) constitute major hydrogen donors for oxidative/reductive bio …
phosphate (NADPH) constitute major hydrogen donors for oxidative/reductive bio …