Synthetic biology: bottom-up assembly of molecular systems

S Hirschi, TR Ward, WP Meier, DJ Müller… - Chemical …, 2022 - ACS Publications
The bottom-up assembly of biological and chemical components opens exciting
opportunities to engineer artificial vesicular systems for applications with previously unmet …

Protein engineering design from directed evolution to de novo synthesis

W **ong, B Liu, Y Shen, K **g, TR Savage - Biochemical Engineering …, 2021 - Elsevier
With the development of enzyme engineering technology, green enzyme catalysis is widely
poised to replace traditional chemical catalysis enabling high enantioselectivity and yielding …

Baeyer–Villiger monooxygenases: tunable oxidative biocatalysts

MJLJ Fürst, A Gran-Scheuch, FS Aalbers… - ACS …, 2019 - ACS Publications
Pollution, accidents, and misinformation have earned the pharmaceutical and chemical
industry a poor public reputation, despite their undisputable importance to society …

Hot spots for the protein engineering of Baeyer-Villiger monooxygenases

K Balke, A Beier, UT Bornscheuer - Biotechnology advances, 2018 - Elsevier
Abstract Baeyer-Villiger monooxygenases (BVMOs) are versatile biocatalysts for the
conversion of ketones to lactones or esters while also being able to efficiently oxidize …

Shifting redox reaction equilibria on demand using an orthogonal redox cofactor

D Aspacio, Y Zhang, Y Cui, E Luu, E King… - Nature Chemical …, 2024 - nature.com
Nature's two redox cofactors, nicotinamide adenine dinucleotide (NAD+) and nicotinamide
adenine dinucleotide phosphate (NADP+), are held at different reduction potentials, driving …

Protein engineering for nicotinamide coenzyme specificity in oxidoreductases: attempts and challenges

AM Chánique, LP Parra - Frontiers in microbiology, 2018 - frontiersin.org
Oxidoreductases are ubiquitous enzymes that catalyze an extensive range of chemical
reactions with great specificity, efficiency, and selectivity. Most oxidoreductases are …

Mutations Increasing Cofactor Affinity, Improve Stability and Activity of a Baeyer–Villiger Monooxygenase

HR Mansouri, O Gracia Carmona, J Jodlbauer… - ACS …, 2022 - ACS Publications
The typically low thermodynamic and kinetic stability of enzymes is a bottleneck for their
application in industrial synthesis. Baeyer–Villiger monooxygenases, which oxidize ketones …

Switching cofactor dependence of 7β-hydroxysteroid dehydrogenase for cost-effective production of ursodeoxycholic acid

ZN You, Q Chen, SC Shi, MM Zheng, J Pan… - Acs …, 2018 - ACS Publications
Dehydrogenases are widely employed as biocatalysts for the production of optically pure
chemicals under mild conditions. Most dehydrogenases are nicotinamide cofactor (NADPH …

Design and engineering of whole-cell biocatalytic cascades for the valorization of fatty acids

JW Song, JH Seo, DK Oh, UT Bornscheuer… - Catalysis Science & …, 2020 - pubs.rsc.org
One of the key factors to construct a productive whole-cell biocatalytic cascade may include
the design of the biotransformation pathways, discovery and engineering of the cascade …

Engineering a Formate Dehydrogenase for NADPH Regeneration

W Ma, Q Geng, C Chen, YC Zheng, HL Yu… - Chembiochem, 2023 - Wiley Online Library
Nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide
phosphate (NADPH) constitute major hydrogen donors for oxidative/reductive bio …