A compendium of single extracellular vesicle flow cytometry

JA Welsh, GJA Arkesteijn, M Bremer… - Journal of …, 2023 - Wiley Online Library
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …

Review on the recent progress in the development of fluorescent probes targeting enzymes

AA Rajapaksha, YX Fu, WY Guo, SY Liu… - … and Applications in …, 2021 - iopscience.iop.org
Enzymes are very important for biological processes in a living being, performing similar or
multiple tasks in and out of cells, tissues and other organisms at a particular location. The …

Membrane vesicles in Acidithiobacillia class extreme acidophiles: influence on collective behaviors of 'Fervidacidithiobacillus caldus'

S Rossoni, S Beard, MI Segura-Bidermann… - Frontiers in …, 2024 - frontiersin.org
Membrane vesicles (MVs) are envelope-derived extracellular sacs that perform a broad
diversity of physiological functions in bacteria. While considerably studied in pathogenic …

[HTML][HTML] Imaging flow cytometry challenges the usefulness of classically used extracellular vesicle labeling dyes and qualifies the novel dye Exoria for the labeling of …

T Tertel, M Schoppet, O Stambouli, A Al-Jipouri… - Cytotherapy, 2022 - Elsevier
Abstract Background aims Extracellular vesicles (EVs) are involved in mediating intercellular
communication processes. An important goal within the EV field is the study of the …

NeuM: A Neuron‐Selective Probe Incorporates into Live Neuronal Membranes via Enhanced Clathrin‐Mediated Endocytosis in Primary Neurons

Y Sung, L Gotina, KH Kim, JY Lee… - Angewandte Chemie …, 2024 - Wiley Online Library
The development of a small‐molecule probe designed to selectively target neurons would
enhance the exploration of intricate neuronal structures and functions. Among such probes …

Silica-coated gold nanorods with hydrophobic modification show both enhanced two-photon fluorescence and ultrasound drug release

EN Mueller, TB Alina, SD Curry, S Ganguly… - Journal of Materials …, 2022 - pubs.rsc.org
Hydrophobically-modified silica-coated gold nanorods are presented here as multifunctional
theranostic agents. A single modification both increases two-photon fluorescence and …

Diffusion of lipid nanovesicles bound to a lipid membrane is associated with the partial-slip boundary condition

E Olsén, S Jõemetsa, A González, P Joyce… - Nano …, 2021 - ACS Publications
During diffusion of nanoparticles bound to a cellular membrane by ligand–receptor pairs, the
distance to the laterally mobile interface is sufficiently short for their motion to depend not …

[HTML][HTML] A lipid transfer protein ensures nematode cuticular impermeability

FN Njume, A Razzauti, M Soler, V Perschin, G Fazeli… - Iscience, 2022 - cell.com
The cuticle of C. elegans is impermeable to chemicals, toxins, and pathogens. However,
increased permeability is a desirable phenotype because it facilitates chemical uptake …

Self-quenching behavior of a fluorescent probe incorporated within lipid membranes explored using electrophoresis and fluorescence lifetime imaging microscopy

SA Meredith, Y Kusunoki, SD Connell… - The Journal of …, 2023 - ACS Publications
Fluorescent probes are useful in biophysics research to assess the spatial distribution,
mobility, and interactions of biomolecules. However, fluorophores can undergo “self …

Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles

G Dhanawat, M Dey, A Singh, N Parveen - ACS omega, 2024 - ACS Publications
We demonstrate rapid membrane mixing between GUVs of pure lipid compositions and
membrane vesicles (MVs) isolated from the plasma membrane of Vero cells, resulting in the …