Physicochemical stability of monoclonal antibodies: a review

Y Le Basle, P Chennell, N Tokhadze, A Astier… - Journal of …, 2020 - Elsevier
Monoclonal antibodies (mAbs) are subject to instability issues linked to their protein nature.
In this work, we review the different mechanisms that can be linked to monoclonal antibodies …

Auristatin antibody drug conjugate physical instability and the role of drug payload

YT Adem, KA Schwarz, E Duenas… - Bioconjugate …, 2014 - ACS Publications
The conjugation of hydrophobic cytotoxic agents such as monomethyl auristatin E (MMAE)
to the interchain sulfhydryl groups of monoclonal antibodies (Mabs) through a protease …

Small amine molecules: solvent design toward facile improvement of protein stability against aggregation and inactivation

K Shiraki, S Tomita, N Inoue - Current pharmaceutical …, 2016 - ingentaconnect.com
Proteins are prone to inactivation in aqueous solutions because chemical modification and
aggregation usually occur, particularly at high temperature. This review focuses on the …

Enhancing protein stability under stress: osmolyte-based deep eutectic solvents as a biocompatible and robust stabilizing medium for lysozyme under heat and cold …

A Damjanović, M Logarušić, LM Tumir… - Physical Chemistry …, 2024 - pubs.rsc.org
In biomedical and biotechnological domains, liquid protein formulations are vital tools,
offering versatility across various fields. However, maintaining protein stability in a liquid …

Agarose native gel electrophoresis analysis of thermal aggregation controlled by Hofmeister series

Y Tomioka, R Sato, R Takahashi, S Nagatoishi… - Biophysical …, 2023 - Elsevier
The effects of salting-in and salting-out salts defined by Hofmeister series on the solution
state of bovine serum albumin (BSA) in 50 mM Tris-HCl buffer at pH 7.4 before and after …

Thermodynamic and fluorescence analyses to determine mechanisms of IgG1 stabilization and destabilization by arginine

M Fukuda, D Kameoka, T Torizawa, S Saitoh… - Pharmaceutical …, 2014 - Springer
Purpose To investigate mechanisms governing the stabilization and destabilization of
immunoglobulin (IgG1) by arginine (Arg). Methods The effects of Arg on the aggregation …

Protein-associated cation clusters in aqueous arginine solutions and their effects on protein stability and size

V Vagenende, AX Han, M Mueller… - ACS chemical biology, 2013 - ACS Publications
Arginine is one of the most prominent residues in protein interactions, and arginine
hydrochloride is widely used as an additive in protein solutions because of its exceptional …

Inhibition of protein aggregation by zwitterionic polymer-based core-shell nanogels

R Rajan, K Matsumura - Scientific reports, 2017 - nature.com
Protein aggregation is a process by which misfolded proteins polymerizes into aggregates
and forms fibrous structures with a β-sheet conformation, known as amyloids. It is an …

Arginine prevents thermal aggregation of hen egg white proteins

T Hong, K Iwashita, A Handa, K Shiraki - Food Research International, 2017 - Elsevier
The control of aggregation and solubilization of hen egg white protein (HEWP) is an
important issue for industrial applications of one of the most familiar food protein sources …

Arginine to lysine mutations increase the aggregation stability of a single-chain variable fragment through unfolded-state interactions

JI Austerberry, A Thistlethwaite, K Fisher… - Biochemistry, 2019 - ACS Publications
Increased protein solubility is known to correlate with an increase in the proportion of lysine
over arginine residues. Previous work has shown that the aggregation propensity of a single …