Unveiling microbial diversity: harnessing long-read sequencing technology
Long-read sequencing has recently transformed metagenomics, enhancing strain-level
pathogen characterization, enabling accurate and complete metagenome-assembled …
pathogen characterization, enabling accurate and complete metagenome-assembled …
Unraveling metagenomics through long-read sequencing: A comprehensive review
The study of microbial communities has undergone significant advancements, starting from
the initial use of 16S rRNA sequencing to the adoption of shotgun metagenomics. However …
the initial use of 16S rRNA sequencing to the adoption of shotgun metagenomics. However …
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect …
Abstract Background Oxford Nanopore Technologies (ONT) offers an accessible platform for
long-read sequencing, which improves the reconstruction of genomes and helps to resolve …
long-read sequencing, which improves the reconstruction of genomes and helps to resolve …
Finding the right fit: evaluation of short-read and long-read sequencing approaches to maximize the utility of clinical microbiome data
JL Gehrig, DM Portik, MD Driscoll… - Microbial …, 2022 - microbiologyresearch.org
A long-standing challenge in human microbiome research is achieving the taxonomic and
functional resolution needed to generate testable hypotheses about the gut microbiota's …
functional resolution needed to generate testable hypotheses about the gut microbiota's …
The application of metagenomics to study microbial communities and develop desirable traits in fermented foods
The microbial communities present within fermented foods are diverse and dynamic,
producing a variety of metabolites responsible for the fermentation processes, imparting …
producing a variety of metabolites responsible for the fermentation processes, imparting …
Comparative analysis of metagenomic classifiers for long-read sequencing datasets
Background Long reads have gained popularity in the analysis of metagenomics data.
Therefore, we comprehensively assessed metagenomics classification tools on the species …
Therefore, we comprehensively assessed metagenomics classification tools on the species …
Amplicon-based microbiome profiling: from second-to third-generation sequencing for higher taxonomic resolution
The 16S rRNA amplicon-based sequencing approach represents the most common and
cost-effective strategy with great potential for microbiome profiling. The use of second …
cost-effective strategy with great potential for microbiome profiling. The use of second …
Refining sampling efforts for fish diversity assessment in subtropical urban estuarine and oceanic waters using environmental DNA with multiple primers
CM How, JCH Ip, D Deconinck, M Zhao… - Environmental …, 2024 - Wiley Online Library
The environmental DNA (eDNA) approach is an emerging tool for monitoring marine
biodiversity. However, the sampling effort needs optimization according to the site …
biodiversity. However, the sampling effort needs optimization according to the site …
Analysis of metagenomic data
Metagenomics has revolutionized our understanding of microbial communities, offering
unprecedented insights into their genetic and functional diversity across Earth's diverse …
unprecedented insights into their genetic and functional diversity across Earth's diverse …
Evaluating metagenomics and targeted approaches for diagnosis and surveillance of viruses
S Buddle, L Forrest, N Akinsuyi, LM Martin Bernal… - Genome Medicine, 2024 - Springer
Background Metagenomics is a powerful approach for the detection of unknown and novel
pathogens. Workflows based on Illumina short-read sequencing are becoming established …
pathogens. Workflows based on Illumina short-read sequencing are becoming established …