The historical reliance of biological research on the use of animal models has sometimes
made it challenging to address questions that are specific to the understanding of human …

Epigenetic research has accelerated rapidly in the twenty-first century, generating justified
excitement and hope, but also a degree of hype. Here we review how the field has evolved …

Organoids bridge the gap between 2D cell lines and in vivo studies. With their 3D
organization and cellular heterogeneity, adult stem cell-derived organoids closely resemble …

Objective Gastric cancer is the second leading cause of cancer-related deaths and the fifth
most common malignancy worldwide. In this study, human and mouse gastric cancer …

Optimization of CRISPR/Cas9-mediated genome engineering has resulted in base editors
that hold promise for mutation repair and disease modeling. Here, we demonstrate the …

METTL3 and METTL14 are two components that form the core heterodimer of the main RNA
m6A methyltransferase complex (MTC) that installs m6A. Surprisingly, depletion of METTL3 …

Adenine base editing (ABE) enables enzymatic conversion from AT into GC base pairs. ABE
holds promise for clinical application, as it does not depend on the introduction of double …

Background Organoids are in vitro three-dimensional structures that can be grown from
patient tissue. Head and neck cancer (HNC) is a collective term used for multiple tumor …

FBXW7 is an E3 ubiquitin ligase that targets proteins for proteasome-mediated degradation
and is mutated in various cancer types. Here, we use CRISPR base editors to introduce …

In mice, intestinal tuft cells have been described as a long-lived, postmitotic cell type. Two
distinct subsets have been identified: tuft-1 and tuft-2 (ref.). By combining analysis of primary …