Glycoproteomics
Protein glycosylation involves the co-translational or post-translational addition of glycans to
proteins and is a crucial protein modification in health and disease. The aim of …
proteins and is a crucial protein modification in health and disease. The aim of …
Analytical and biochemical perspectives of protein O-GlcNAcylation
Protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) is a
unique monosaccharide modification discovered in the early 1980s. With the technological …
unique monosaccharide modification discovered in the early 1980s. With the technological …
Optimal Dissociation Methods Differ for N- and O-Glycopeptides
Site-specific characterization of glycosylation requires intact glycopeptide analysis, and
recent efforts have focused on how to best interrogate glycopeptides using tandem mass …
recent efforts have focused on how to best interrogate glycopeptides using tandem mass …
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins
Mucin domains are densely O-glycosylated modular protein domains that are found in a
wide variety of cell surface and secreted proteins. Mucin-domain glycoproteins are known to …
wide variety of cell surface and secreted proteins. Mucin-domain glycoproteins are known to …
Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis
Protein glycosylation is a highly important, yet poorly understood protein post-translational
modification. Thousands of possible glycan structures and compositions create potential for …
modification. Thousands of possible glycan structures and compositions create potential for …
Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics
Protein glycosylation, a complex and heterogeneous post-translational modification that is
frequently dysregulated in disease, has been difficult to analyse at scale. Here we report a …
frequently dysregulated in disease, has been difficult to analyse at scale. Here we report a …
Predicting glycan structure from tandem mass spectrometry via deep learning
Glycans constitute the most complicated post-translational modification, modulating protein
activity in health and disease. However, structural annotation from tandem mass …
activity in health and disease. However, structural annotation from tandem mass …
Maturing glycoproteomics technologies provide unique structural insights into the N-glycoproteome and its regulation in health and disease
The glycoproteome remains severely understudied because of significant analytical
challenges associated with glycoproteomics, the system-wide analysis of intact …
challenges associated with glycoproteomics, the system-wide analysis of intact …
The benefits of hybrid fragmentation methods for glycoproteomics
Glycosylation is an important and variable protein modification that can have a profound
effect on the physiological characteristics of the substrate, warranting careful examination in …
effect on the physiological characteristics of the substrate, warranting careful examination in …
NIST interlaboratory study on glycosylation analysis of monoclonal antibodies: comparison of results from diverse analytical methods
MLA De Leoz, DL Duewer, A Fung, L Liu… - Molecular & Cellular …, 2020 - ASBMB
Glycosylation is a topic of intense current interest in the development of biopharmaceuticals
because it is related to drug safety and efficacy. This work describes results of an …
because it is related to drug safety and efficacy. This work describes results of an …