Fiber photometry is a key technique for characterizing brain-behavior relationships in vivo.
Initially, it was primarily used to report calcium dynamics as a proxy for neural activity via …

Over the past 20 years, protein engineering has been extensively used to improve and
modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting …

Effective protein quality control (PQC), essential for cellular health, relies on spatial
sequestration of misfolded proteins into defined inclusions. Here we reveal the coordination …

The development of whole-body imaging at single-cell resolution enables system-level
approaches to studying cellular circuits in organisms. Previous clearing methods focused on …

Ultrafast processes in light-absorbing proteins have been implicated in the primary step in
the light-to-energy conversion and the initialization of photoresponsive biological functions …

The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

Time-dependent density-functional theory (TDDFT) describes the quantum dynamics of
interacting electronic many-body systems formally exactly and in a practical and efficient …

Organism-level systems biology aims to identify, analyze, control and design cellular circuits
in organisms. Many experimental and computational approaches have been developed …

Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Efficient collection and detection of fluorescence coupled with careful minimization of
background from impurities and Raman scattering now enable routine optical microscopy …