Recombinant protein expression: challenges in production and folding related matters

A Beygmoradi, A Homaei, R Hemmati… - International Journal of …, 2023 - Elsevier
Protein folding is a biophysical process by which proteins reach a specific three-dimensional
structure. The amino acid sequence of a polypeptide chain contains all the information …

Factors involved in heterologous expression of proteins in E. coli host

M Pouresmaeil, S Azizi-Dargahlou - Archives of Microbiology, 2023 - Springer
The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …

Enhancing cyclotide bioproduction: harnessing biological synthesis methods and various expression systems for large-scale manufacturing

MS Taghizadeh, A Niazi… - Critical Reviews in …, 2024 - Taylor & Francis
Peptide-based medications hold immense potential in addressing a wide range of human
disorders and discomforts. However, their widespread utilization encounters two major …

Exigency of Plant-Based Vaccine against COVID-19 Emergence as Pandemic Preparedness

A Chattopadhyay, AAK Jailani, B Mandal - Vaccines, 2023 - mdpi.com
After two years since the declaration of COVID-19 as a pandemic by the World Health
Organization (WHO), more than six million deaths have occurred due to SARS-CoV-2 …

Enhancing leptospirosis control with nanosensing technology: A critical analysis

P Suwannin, K Jangpatarapongsa, D Polpanich… - … and Infectious Diseases, 2024 - Elsevier
Leptospirosis is a serious health problem in tropical areas; thus, animals shed leptospires in
the environment. Humans are accidental hosts infected through exposure to contaminating …

Soluble Human Angiotensin- Converting Enzyme 2 as a Potential Therapeutic Tool for COVID-19 is Produced at High Levels In Nicotiana benthamiana Plant With …

T Mamedov, I Gurbuzaslan, D Yuksel, M Ilgin… - Frontiers in Plant …, 2021 - frontiersin.org
The coronavirus disease 2019 (COVID-19) pandemic, which is caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread to more than 222 …

[HTML][HTML] Model-assisted CRISPRi/a library screening reveals central carbon metabolic targets for enhanced recombinant protein production in yeast

X Chen, F Li, X Li, M Otto, Y Chen, V Siewers - Metabolic Engineering, 2025 - Elsevier
Production of recombinant proteins is regarded as an important breakthrough in the field of
biomedicine and industrial biotechnology. Due to the complexity of the protein secretory …

Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle …

C Casonato Melo, AC Fux, M Himly, NG Bastús… - International Journal of …, 2023 - mdpi.com
Endotoxins or lipopolysaccharides (LPS), found in the outer membrane of Gram-negative
bacterial cell walls, can stimulate the human innate immune system, leading to life …

Postbiotic emissaries: a comprehensive review on the bioprospecting and production of bioactive compounds by Enterococcus species

A Chaudhary, N Prajapati, A Prajapati… - … Journal of Food …, 2024 - Wiley Online Library
Enterococcus species have been acknowledged for their diverse metabolic capabilities,
resulting in a wide array of bioactive compounds that show promise for therapeutic use. This …

Alternative secretory signal sequences for recombinant protein production in Pichia pastoris

M Karaoğlan - Enzyme and Microbial Technology, 2023 - Elsevier
Extracellular protein production is primarily preferred to facilitate the downstream processes
in recombinant protein production. Secretion of recombinant proteins is mediated by the …