Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics

S Qin, W **ao, C Zhou, Q Pu, X Deng, L Lan… - Signal transduction and …, 2022 - nature.com
Pseudomonas aeruginosa (P. aeruginosa) is a Gram-negative opportunistic pathogen that
infects patients with cystic fibrosis, burn wounds, immunodeficiency, chronic obstructive …

Controlling and enhancing CRISPR systems

H Shivram, BF Cress, GJ Knott, JA Doudna - Nature chemical biology, 2021 - nature.com
Many bacterial and archaeal organisms use clustered regularly interspaced short
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

LB Harrington, D Burstein, JS Chen, D Paez-Espino… - Science, 2018 - science.org
CRISPR-Cas systems provide microbes with adaptive immunity to infectious nucleic acids
and are widely employed as genome editing tools. These tools use RNA-guided Cas …

Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing

BA Adler, T Hessler, BF Cress, A Lahiri, VK Mutalik… - Nature …, 2022 - nature.com
CRISPR-Cas13 proteins are RNA-guided RNA nucleases that defend against incoming
RNA and DNA phages by binding to complementary target phage transcripts followed by …

Anti-CRISPR: discovery, mechanism and function

A Pawluk, AR Davidson, KL Maxwell - Nature Reviews Microbiology, 2018 - nature.com
CRISPR–Cas adaptive immune systems are widespread among bacteria and archaea.
Recent studies have shown that these systems have minimal long-term evolutionary effects …

Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies

ND Marino, R Pinilla-Redondo, B Csörgő… - Nature …, 2020 - nature.com
Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-
associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have …

Spatiotemporal control of CRISPR/Cas9 gene editing

C Zhuo, J Zhang, JH Lee, J Jiao, D Cheng… - … and Targeted Therapy, 2021 - nature.com
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein
9 (CRISPR/Cas9) gene editing technology, as a revolutionary breakthrough in genetic …

[HTML][HTML] CRISPR-Cas systems: Overview, innovations and applications in human disease research and gene therapy

Y Xu, Z Li - Computational and structural biotechnology journal, 2020 - Elsevier
Genome editing is the modification of genomic DNA at a specific target site in a wide variety
of cell types and organisms, including insertion, deletion and replacement of DNA, resulting …

A compact, high-accuracy Cas9 with a dinucleotide PAM for in vivo genome editing

A Edraki, A Mir, R Ibraheim, I Gainetdinov, Y Yoon… - Molecular cell, 2019 - cell.com
CRISPR-Cas9 genome editing has transformed biotechnology and therapeutics. However,
in vivo applications of some Cas9s are hindered by large size (limiting delivery by adeno …

Inhibitors of bacterial immune systems: discovery, mechanisms and applications

D Mayo-Muñoz, R Pinilla-Redondo… - Nature Reviews …, 2024 - nature.com
To contend with the diversity and ubiquity of bacteriophages and other mobile genetic
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …