Homeostatic and pathological phenomena often affect multiple organs across the whole
organism. Tissue clearing methods, together with recent advances in microscopy, have …

Tissue clearing increases the transparency of late developmental stages and enables deep
imaging in fixed organisms. Successful implementation of these methodologies requires a …

The accumulation of deleterious mitochondrial DNA (∆ mtDNA) causes inherited
mitochondrial diseases and ageing-associated decline in mitochondrial functions such as …

Light microscopy enables researchers to observe cellular mechanisms with high spatial and
temporal resolution. However, the increasing complexity of current imaging technologies …

The combination of optical tissue transparency with immunofluorescence allows the
molecular characterization of biological tissues in 3D. However, adult human organs are …

Recent advances in single-molecule imaging of mRNAs in fixed and living cells have
enabled the lives of mRNAs to be studied with unprecedented spatial and temporal detail …

Revealing the 3D composition of intact tissue specimens is essential for understanding cell
and organ biology in health and disease. State-of-the-art 3D microscopy techniques aim to …

Abstract Analysis of three-dimensional patient specimens is gaining increasing relevance for
understanding the principles of tissue structure as well as the biology and mechanisms …

Time-lapse fluorescence microscopy is key to unraveling biological development and
function; however, living systems, by their nature, permit only limited interrogation and …

Light‐sheet microscopy (LSM) enables us to strengthen the understanding of cardiac
development, injury, and regeneration in mammalian models. This emerging technique …