Glycosylation plays a pivotal role in tuning the folding and function of proteins. Because
most human therapeutic proteins are glycosylated, understanding and controlling …

Protein glycosylation, which involves the addition of carbohydrate chains to amino acid side
chains, imparts essential properties to proteins, offering immense potential in synthetic …

Cell-free gene expression systems are used in numerous applications, including medicine
making, diagnostics, and educational kits. Accurate quantification of nonfluorescent proteins …

N-linked glycosylation plays a key role in the efficacy of many therapeutic proteins. One
limitation to the bacterial glycoengineering of human N-linked glycans is the difficulty of …

The important roles that protein glycosylation plays in modulating the activities and efficacies
of protein therapeutics have motivated the development of synthetic glycosylation systems in …

Effective diagnostics and therapeutics are foundational to modern medical technology. As
the drug modality transitions from traditional small-molecule drugs to more intricate protein …

The fundamental aspect of cell-free synthesis systems is the in vitro transcription-translation
process. By artificially providing the components required for protein expression, in vitro …

The SARS-CoV-2 pandemic highlighted the urgent need for biomanufacturing paradigms
that are robust and fast. Here, we demonstrate the rapid process development and scalable …

Cell-free gene expression (CFE) systems enable transcription and translation using crude
cellular extracts, offering a versatile platform for synthetic biology by eliminating the need to …

Cell-free protein synthesis (CFPS), whereby cell lysates are used to produce proteins from a
genetic template, has matured as an attractive alternative to standard biomanufacturing …