Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek
observed living microorganisms and cells in 1674 using his light microscope. A spectrum of …

Photosynthesis begins when a network of pigment–protein complexes captures solar energy
and transports it to the reaction center, where charge separation occurs. When necessary …

Cryo-soft X-ray tomography is an imaging technique that addresses the need for mesoscale
imaging of cellular ultrastructure of relatively thick samples without the need for staining or …

Mitochondrial fission is important for organelle transport, quality control and apoptosis.
Changes to the fission process can result in a wide variety of neurological diseases. In …

In recent years correlative microscopy, combining the power and advantages of different
imaging system, eg, light, electrons, X-ray, NMR, etc., has become an important tool for …

Water-window x-ray microscopy allows two-and three-dimensional (2D and 3D) imaging of
intact unstained cells in their cryofixed near-native state with unique contrast and high …

Highlights•Soft X-ray tomography (SXT) is a fast, quantitative method that is easy to
perform.•SXT visualizes fully hydrated, intact cells without the use of labeling or …

Soft x-ray tomography (SXT) is increasingly being recognized as a valuable method for
visualizing and quantifying the ultrastructure of cryopreserved cells. Here, we describe the …

Cryogenic fluorescent light microscopy of flash-frozen cells stands out by artifact-free fixation
and very little photobleaching of the fluorophores used. To attain the highest level of …

Lytic infection with herpes simplex virus type 1 (HSV-1) induces profound modification of the
cell nucleus including formation of a viral replication compartment and chromatin …