Protein design: From the aspect of water solubility and stability

R Qing, S Hao, E Smorodina, D **, A Zalevsky… - Chemical …, 2022 - ACS Publications
Water solubility and structural stability are key merits for proteins defined by the primary
sequence and 3D-conformation. Their manipulation represents important aspects of the …

The styrene–maleic acid copolymer: a versatile tool in membrane research

JM Dörr, S Scheidelaar, MC Koorengevel… - European Biophysics …, 2016 - Springer
A new and promising tool in membrane research is the detergent-free solubilization of
membrane proteins by styrene–maleic acid copolymers (SMAs). These amphipathic …

The TOPCONS web server for consensus prediction of membrane protein topology and signal peptides

KD Tsirigos, C Peters, N Shu, L Käll… - Nucleic acids …, 2015 - academic.oup.com
Abstract TOPCONS (http://topcons. net/) is a widely used web server for consensus
prediction of membrane protein topology. We hereby present a major update to the server …

OPM database and PPM web server: resources for positioning of proteins in membranes

MA Lomize, ID Pogozheva, H Joo… - Nucleic acids …, 2012 - academic.oup.com
Abstract The Orientations of Proteins in Membranes (OPM) database is a curated web
resource that provides spatial positions of membrane-bound peptides and proteins of known …

Characterization of lipid–protein interactions and lipid-mediated modulation of membrane protein function through molecular simulation

MP Muller, T Jiang, C Sun, M Lihan, S Pant… - Chemical …, 2019 - ACS Publications
The cellular membrane constitutes one of the most fundamental compartments of a living
cell, where key processes such as selective transport of material and exchange of …

CCTOP: a Consensus Constrained TOPology prediction web server

L Dobson, I Reményi, GE Tusnády - Nucleic acids research, 2015 - academic.oup.com
Abstract The Consensus Constrained TOPology prediction (CCTOP; http://cctop. enzim. ttk.
mta. hu) server is a web-based application providing transmembrane topology prediction. In …

Mechanistic landscape of membrane-permeabilizing peptides

S Guha, J Ghimire, E Wu, WC Wimley - Chemical reviews, 2019 - ACS Publications
Membrane permeabilizing peptides (MPPs) are as ubiquitous as the lipid bilayer
membranes they act upon. Produced by all forms of life, most membrane permeabilizing …

[HTML][HTML] Surface plasmon resonance spectroscopy for characterisation of membrane protein–ligand interactions and its potential for drug discovery

SG Patching - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2014 - Elsevier
Surface plasmon resonance (SPR) spectroscopy is a rapidly develo** technique for the
study of ligand binding interactions with membrane proteins, which are the major molecular …

Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes

A Krogh, B Larsson, G Von Heijne… - Journal of molecular …, 2001 - Elsevier
We describe and validate a new membrane protein topology prediction method, TMHMM,
based on a hidden Markov model. We present a detailed analysis of TMHMM's performance …

Mass spectrometry methods for measuring protein stability

DD Vallejo, C Rojas Ramírez, KF Parson, Y Han… - Chemical …, 2022 - ACS Publications
Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …