[HTML][HTML] Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

W Ahmed, SL Simpson, PM Bertsch, K Bibby… - Science of the Total …, 2022 - Elsevier
Wastewater surveillance for pathogens using reverse transcription-polymerase chain
reaction (RT-PCR) is an effective and resource-efficient tool for gathering community-level …

Schrödinger's microbes: tools for distinguishing the living from the dead in microbial ecosystems

JB Emerson, RI Adams, CMB Román, B Brooks… - Microbiome, 2017 - Springer
While often obvious for macroscopic organisms, determining whether a microbe is dead or
alive is fraught with complications. Fields such as microbial ecology, environmental health …

The environmental microbiology minimum information (EMMI) guidelines: qPCR and dPCR quality and reporting for environmental microbiology

MA Borchardt, AB Boehm, M Salit… - Environmental …, 2021 - ACS Publications
Real-time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) methods
have revolutionized environmental microbiology, yielding quantitative organism-specific …

Comparison of RT-qPCR and RT-dPCR platforms for the trace detection of SARS-CoV-2 RNA in wastewater

W Ahmed, WJM Smith, S Metcalfe, G Jackson… - Acs Es&T …, 2022 - ACS Publications
We compared reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and
RT digital PCR (RT-dPCR) platforms for the trace detection of SARS-CoV-2 RNA in low …

Application of digital PCR for public health-related water quality monitoring

A Tiwari, W Ahmed, S Oikarinen, SP Sherchan… - Science of the Total …, 2022 - Elsevier
Digital polymerase chain reaction (dPCR) is emerging as a reliable platform for quantifying
microorganisms in the field of water microbiology. This paper reviews the fundamental …

Droplet microfluidics for microbiology: techniques, applications and challenges

TS Kaminski, O Scheler, P Garstecki - Lab on a Chip, 2016 - pubs.rsc.org
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …

A framework for standardized qPCR-targets and protocols for quantifying antibiotic resistance in surface water, recycled water and wastewater

I Keenum, K Liguori, J Calarco, BC Davis… - Critical Reviews in …, 2022 - Taylor & Francis
Water environments are increasingly recognized as a conduit for the spread of antibiotic
resistance, but there is need to standardize antibiotic resistance monitoring protocols to …

Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater

M Ciesielski, D Blackwood, T Clerkin… - Journal of Virological …, 2021 - Elsevier
Throughout the COVID-19 global pandemic there has been significant interest and
investment in using Wastewater-Based Epidemiology (WBE) for surveillance of viral …

Microbial indicators of fecal pollution: recent progress and challenges in assessing water quality

DA Holcomb, JR Stewart - Current environmental health reports, 2020 - Springer
Abstract Purpose of Review Fecal contamination of water is a major public health concern.
This review summarizes recent developments and advancements in water quality indicators …

Advancements in detection and removal of antibiotic resistance genes in sludge digestion: A state-of-art review

Z Zhang, X Li, H Liu, A Zamyadi, W Guo, H Wen… - Bioresource …, 2022 - Elsevier
Sludge from wastewater treatment plants can act as a repository and crucial environmental
provider of antibiotic resistance genes (ARGs). Over the past few years, people's knowledge …