Polymerase chain reaction (PCR) is a simple and rapid method that can detect nucleotide
polymorphisms and sequence variation in basic research applications, agriculture, and …

Background: Type 1 long QT syndrome (LQT1) is caused by loss-of-function variants in the
KCNQ1-encoded Kv7. 1 potassium channel α-subunit that is essential for cardiac …

The use of zebrafish in functional genomics and disease modeling has become popular due
to the ease of targeted mutagenesis with genome editing nucleases, ie, zinc finger …

The proposed method is a modified and improved version of the existing “Allele-specific q-
PCR”(ASQ) method for genoty** of single nucleotide polymorphism (SNP) based on …

Human genetics analysis has identified many noncoding SNPs associated with diabetic
traits, but whether and how these variants contribute to diabetes is largely unknown. Here …

Zebrafish (Danio rerio) is a unique model organism at the functional intersection between a
high fecundity and conserved vertebrate physiology while being amenable to a multitude of …

Advances in genome engineering have resulted in the generation of numerous zebrafish
mutant lines. A commonly used method to assess gene expression in the mutants is in situ …

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-
associated protein 9 (Cas9)-mediated genome editing system is widely used for targeted …

Genoty** is the process of determining differences in the genetic make-up of an individual
and comparing it to that of another individual. Focus on the family of chemosensory proteins …

This study developed a simple allele specific (AS) PCR assay for the detection of pathogenic
Vibrio cholerae strains in seafood. A pair of primers,(1) an AS primer targeting the mutation …