Genome-editing technologies: concept, pros, and cons of various genome-editing techniques and bioethical concerns for clinical application

SH Khan - Molecular Therapy-Nucleic Acids, 2019 - cell.com
The traditional healthcare system is at the doorstep for entering into the arena of molecular
medicine. The enormous knowledge and ongoing research have now been able to …

PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures

D Gleditzsch, P Pausch, H Müller-Esparza, A Özcan… - RNA biology, 2019 - Taylor & Francis
Adaptive immunity of prokaryotes is mediated by CRISPR-Cas systems that employ a large
variety of Cas protein effectors to identify and destroy foreign genetic material. The different …

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

W Jiang, D Bikard, D Cox, F Zhang… - Nature biotechnology, 2013 - nature.com
Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–
associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations …

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

MH Larson, LA Gilbert, X Wang, WA Lim… - Nature protocols, 2013 - nature.com
Sequence-specific control of gene expression on a genome-wide scale is an important
approach for understanding gene functions and for engineering genetic regulatory systems …

CRISPR-Cas systems and methods for altering expression of gene products

F Zhang - US Patent 8,697,359, 2014 - Google Patents
The invention provides for systems, methods, and compositions for altering expression of
target gene sequences and related gene products. Provided are vectors and vector systems …

CRISPR-Cas component systems, methods and compositions for sequence manipulation

F Zhang - US Patent 8,795,965, 2014 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
49881125&utm_source= google_patent&utm_medium= platform_link&utm_campaign …

CRISPR-Cas component systems, methods and compositions for sequence manipulation

L Cong, F Zhang - US Patent 8,871,445, 2014 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
49881125&utm_source= google_patent&utm_medium= platform_link&utm_campaign …

CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes

L Cong, F Zhang - US Patent 8,932,814, 2015 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
49883299&utm_source= google_patent&utm_medium= platform_link&utm_campaign …

Engineering of systems, methods and optimized guide compositions for sequence manipulation

F Zhang, L Cong, P Hsu, RAN Fei - US Patent 8,906,616, 2014 - Google Patents
The invention provides for systems, methods, and compositions for manipulation of
sequences and/or activities of target sequences. Provided are vectors and vector systems …

CRISPR-Cas systems and methods for altering expression of gene products

F Zhang - US Patent 8,771,945, 2014 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
50441380&utm_source= google_patent&utm_medium= platform_link&utm_campaign …