[HTML][HTML] Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

W Ahmed, SL Simpson, PM Bertsch, K Bibby… - Science of the Total …, 2022 - Elsevier
Wastewater surveillance for pathogens using reverse transcription-polymerase chain
reaction (RT-PCR) is an effective and resource-efficient tool for gathering community-level …

Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives

T Dong, M Wang, J Liu, P Ma, S Pang, W Liu… - Chemical Science, 2023 - pubs.rsc.org
The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
has induced severe public healthcare issues and weakened the global economy …

SARS-CoV-2 E gene variant alters analytical sensitivity characteristics of viral detection using a commercial reverse transcription-PCR assay

S Tahan, BA Parikh, L Droit, MA Wallace… - Journal of clinical …, 2021 - journals.asm.org
Diagnostic assays for detecting severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) are essential for patient management, infection prevention, and the public health …

Decentralizing cell-free RNA sensing with the use of low-cost cell extracts

A Arce, F Guzman Chavez, C Gandini, J Puig… - … in Bioengineering and …, 2021 - frontiersin.org
Cell-free gene expression systems have emerged as a promising platform for field-deployed
biosensing and diagnostics. When combined with programmable toehold switch-based RNA …

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection

TGW Graham, C Dugast-Darzacq, GM Dailey… - PloS one, 2021 - journals.plos.org
Re-opening of communities in the midst of the ongoing COVID-19 pandemic has ignited
new waves of infections in many places around the world. Mitigating the risk of reopening …

Accessible LAMP-enabled rapid test (ALERT) for detecting SARS-CoV-2

A Bektaş, MF Covington, G Aidelberg, A Arce, T Matute… - Viruses, 2021 - mdpi.com
The coronavirus disease 2019 (COVID-19) pandemic has highlighted bottlenecks in large-
scale, frequent testing of populations for infections. Polymerase chain reaction (PCR)-based …

Homebrew reagents for low-cost RT-LAMP

T Matute, I Nuñez, M Rivera, J Reyes… - Journal of …, 2021 - pmc.ncbi.nlm.nih.gov
Reverse transcription–loop-mediated isothermal amplification (RT-LAMP) has gained
popularity for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV …

Analytical sensitivity and clinical performance of" COVID-19 RT-PCR Real TM FAST (CY5)(ATGen, Uruguay) and" ECUGEN SARS-CoV-2 RT-qPCR"(UDLA …

B Freire-Paspuel, D Morales-Jadan… - PLoS Neglected …, 2022 - journals.plos.org
Background Dozens of commercial RT-qPCR kits for SARS-CoV-2 detection are available
with or without Emergency Use Authorization (EUA) by FDA or other regulatory agencies …

Characteristics of patients with Coronavirus Disease 2019 (COVID-19) and seasonal influenza at time of hospital admission: a single center comparative study

P Sieber, D Flury, S Güsewell, WC Albrich… - BMC Infectious …, 2021 - Springer
Background In the future, co-circulation of severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) and influenza viruses A/B is likely. From a clinical point of view, differentiation …

Evaluation and Clinical Validation of Guanidine‐Based Inactivation Transport Medium for Preservation of SARS‐CoV‐2

HL Wiraswati, S Gaffar, S Ekawardhani… - Advances in …, 2022 - Wiley Online Library
WHO declared the outbreak of COVID‐19, caused by SARS‐CoV‐2, a pandemic in March
2020. More than 223 million cases and approximately 4.6 million deaths have been …