Nucleic acid aptamers for molecular diagnostics and therapeutics: advances and perspectives

L Li, S Xu, H Yan, X Li, HS Yazd, X Li… - Angewandte Chemie …, 2021‏ - Wiley Online Library
The advent of SELEX (systematic evolution of ligands by exponential enrichment)
technology has shown the ability to evolve artificial ligands with affinity and specificity able to …

Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease

EWM Ng, DT Shima, P Calias… - Nature reviews drug …, 2006‏ - nature.com
Aptamers are oligonucleotide ligands that are selected for high-affinity binding to molecular
targets. Pegaptanib sodium (Macugen; Eyetech Pharmaceuticals/Pfizer) is an RNA aptamer …

SELEX—A (r) evolutionary method to generate high-affinity nucleic acid ligands

R Stoltenburg, C Reinemann, B Strehlitz - Biomolecular engineering, 2007‏ - Elsevier
SELEX stands for systematic evolution of ligands by exponential enrichment. This method,
described primarily in 1990 [Ellington, AD, Szostak, JW, 1990. In vitro selection of RNA …

Nucleic acid based molecular devices

Y Krishnan, FC Simmel - Angewandte Chemie International …, 2011‏ - Wiley Online Library
In biology, nucleic acids are carriers of molecular information: DNA's base sequence stores
and imparts genetic instructions, while RNA's sequence plays the role of a messenger and a …

The chemical biology of aptamers

G Mayer - Angewandte Chemie International Edition, 2009‏ - Wiley Online Library
Taking a strand: Aptamers are small single‐stranded oligonucleotides that fold into a well‐
defined 3D structure and interact with high affinity and specificity with their target molecules …

Aptamers and the next generation of diagnostic reagents

V Thiviyanathan, DG Gorenstein - PROTEOMICS–Clinical …, 2012‏ - Wiley Online Library
Antibodies have been extensively used as capture and detection reagents in diagnostic
applications of proteomics‐based technologies. Proteomic assays need high sensitivity and …

FluMag-SELEX as an advantageous method for DNA aptamer selection

R Stoltenburg, C Reinemann, B Strehlitz - Analytical and bioanalytical …, 2005‏ - Springer
Aptamers are ssDNA or RNA oligonucleotides with very high affinity for their target. They
bind to the target with high selectivity and specificity because of their specific three …

Selection and analytical applications of aptamers

CLA Hamula, JW Guthrie, H Zhang, XF Li… - TrAC Trends in Analytical …, 2006‏ - Elsevier
Advances in systematic evolution of ligands by exponential enrichment (SELEX), a selection
protocol for aptamers, have resulted in increased applications of DNA and RNA aptamers in …

Non-SELEX: selection of aptamers without intermediate amplification of candidate oligonucleotides

MV Berezovski, MU Musheev, AP Drabovich… - Nature protocols, 2006‏ - nature.com
Aptamers are typically selected from libraries of random DNA (or RNA) sequences through
systematic evolution of ligands by exponential enrichment (SELEX), which involves several …

Piperine for treating breast cancer: A review of molecular mechanisms, combination with anticancer drugs, and nanosystems

CR Quijia, M Chorilli - Phytotherapy Research, 2022‏ - Wiley Online Library
Piperine (PIP) is an alkaloid found primarily in Piper longum, and this natural compound has
been shown to exert effects on proliferation and survival against various types of cancer. In …