Membrane proteins play a most important part in metabolism, signaling, cell motility,
transport, development, and many other biochemical and biophysical processes which …

Membrane proteins have long presented a challenge to biochemical and functional studies.
In the absence of a bilayer environment, individual proteins and critical macromolecular …

Bacteria produce a wide range of exopolysaccharides which are synthesized via different
biosynthesis pathways. The genes responsible for synthesis are often clustered within the …

Membrane proteins are involved in numerous vital biological processes, including transport,
signal transduction and the enzymes in a variety of metabolic pathways. Integral membrane …

Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …

When taking up the gauntlet of studying membrane protein functionality, scientists are
provided with a plethora of advantages, which can be exploited for the synthesis of these …

Highlights•Compares and contrasts new affinity methods to dominant conventional
methods.•Explains how the popular His tag, despite its age, still dominates this …

Cell-free protein expression plays an important role in biochemical research. However, only
recent developments led to new methods to rapidly synthesize preparative amounts of …

Liposome display is a novel method for in vitro selection and directed evolution of
membrane proteins. In this approach, membrane proteins of interest are displayed on …

Copolymers of styrene and maleic acid (SMA) have gained great attention as alternatives to
conventional detergents, as they offer decisive advantages for studying membrane proteins …