Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

Although next-generation sequencing (NGS) technology revolutionized sequencing, offering
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …

Motivation Although long-read sequencing technologies can produce genomes with long
contiguity, they suffer from high error rates. Thus, we developed NextPolish, a tool that …

Although originally primarily a system for functional biology, Arabidopsis thaliana has, owing
to its broad geographical distribution and adaptation to diverse environments, developed …

Long nanopore reads are advantageous in de novo genome assembly. However, nanopore
reads usually have broad error distribution and high-error-rate subsequences. Existing error …

In recent years, nanopore technology has become increasingly important in the field of life
science and biomedical research. By embedding a nano-scale hole in a thin membrane and …

Forty years ago the advent of Sanger sequencing was revolutionary as it allowed complete
genome sequences to be deciphered for the first time. A second revolution came when next …

Genomic differences range from single nucleotide differences to complex structural
variations. Current methods typically annotate sequence differences ranging from SNPs to …

We present RaGOO, a reference-guided contig ordering and orienting tool that leverages
the speed and sensitivity of Minimap2 to accurately achieve chromosome-scale assemblies …

Assembling a plant genome is challenging due to the abundance of repetitive sequences,
yet no standard is available to evaluate the assembly of repeat space. LTR retrotransposons …