Environmental DNA metabarcoding: A novel method for biodiversity monitoring of marine fish communities

M Miya - Annual Review of Marine Science, 2022 - annualreviews.org
Environmental DNA (eDNA) is genetic material that has been shed from macroorganisms. It
has received increased attention as an indirect marker for biodiversity monitoring. This …

MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Coral reef ecosystem functioning: eight core processes and the role of biodiversity

SJ Brandl, DB Rasher, IM Côté… - Frontiers in Ecology …, 2019 - Wiley Online Library
Coral reefs are in global decline. Reversing this trend is a primary management objective
but doing so depends on understanding what keeps reefs in desirable states (ie …

Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap

T Cordier, L Alonso‐Sáez… - Molecular …, 2021 - Wiley Online Library
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …

Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity

M Stat, J John, JD DiBattista, SJ Newman… - Conservation …, 2019 - Wiley Online Library
Monitoring communities of fish is important for the management and sustainability of
fisheries and marine ecosystems. Baited remote underwater video systems (BRUVs) are …

A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish

S Zhang, J Zhao, M Yao - Methods in Ecology and Evolution, 2020 - Wiley Online Library
Accurate assessments of fish species diversity and community composition are essential for
understanding fish ecology and conservation management. Environmental DNA (eDNA) …

Calibrating environmental DNA metabarcoding to conventional surveys for measuring fish species richness

ME McElroy, TL Dressler, GC Titcomb… - Frontiers in Ecology …, 2020 - frontiersin.org
The ability to properly identify species present in a landscape is foundational to ecology and
essential for natural resource management and conservation. However, many species are …

Comparing environmental DNA metabarcoding and underwater visual census to monitor tropical reef fishes

A Polanco Fernández, V Marques, F Fopp… - Environmental …, 2021 - Wiley Online Library
Environmental DNA (eDNA) analysis is a revolutionary method to monitor marine
biodiversity from animal DNA traces. Examining the capacity of eDNA to provide accurate …

Environmental DNA metabarcoding studies are critically affected by substrate selection

A Koziol, M Stat, T Simpson, S Jarman… - Molecular Ecology …, 2019 - Wiley Online Library
Effective biomonitoring is critical for driving management outcomes that ensure long‐term
sustainability of the marine environment. In recent years, environmental DNA (eDNA) …

A performance evaluation of targeted eDNA and eDNA metabarcoding analyses for freshwater fishes

J Bylemans, DM Gleeson, RP Duncan… - Environmental …, 2019 - Wiley Online Library
Background The use of environmental DNA analysis has revolutionized biodiversity
monitoring. Initially, eDNA monitoring surveys in aquatic environments utilized a targeted …