The regulated transcription of genes determines cell identity and function. Recent structural
studies have elucidated mechanisms that govern the regulation of transcription by RNA …

The eukaryotic transcription apparatus synthesizes a staggering diversity of RNA molecules.
The labour of nuclear gene transcription is, therefore, divided among multiple DNA …

During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin,
but the mechanism has remained elusive. Using cryo–electron microscopy, we obtained …

The journey of RNA polymerase II (Pol II) as it transcribes a gene is anything but a smooth
ride. Transcript elongation is discontinuous and can be perturbed by intrinsic regulatory …

Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Since the first high-resolution structure of the nucleosome was reported in 1997, the
available information on chromatin structure has increased very rapidly. Here, we review …

In eukaryotes, RNA polymerase (Pol) II transcribes chromatin and must move past
nucleosomes, often resulting in nucleosome displacement. How Pol II unwraps the DNA …

The organization of genomic DNA into nucleosomes profoundly affects all DNA-related
processes in eukaryotes. The histone chaperone known as 'facilitates chromatin …

RNA polymerase II–mediated eukaryotic transcription starts with the assembly of the
preinitiation complex (PIC) on core promoters. The+ 1 nucleosome is well positioned about …

During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the
help of various elongation factors. Here, we show that RNA Pol II achieves efficient …