Online comprehensive two‐dimensional liquid chromatography has become an attractive
option for the analysis of complex nonvolatile samples found in various fields (eg …

Mass spectrometry (MS)–based high-throughput proteomics is the core technique for large-
scale protein characterization. Due to the extreme complexity of proteomes, sophisticated …

Closely related to studying the function of a protein is the analysis of its three‐dimensional
structure and the identification of interaction sites with its binding partners. An alternative …

In-depth MS-based proteomics has necessitated fractionation of either proteins or peptides
or both, often requiring considerable analysis time. Here we employ long liquid …

Proteomic and lipidomic profiling was performed over a time course of acute hepatitis C
virus (HCV) infection in cultured Huh-7.5 cells to gain new insights into the intracellular …

The possible combination of specific physicochemical properties operating at unique sites of
action within cells and tissues has led to considerable uncertainty surrounding nanomaterial …

The enormous complexity, wide dynamic range of relative protein abundances of interest
(over 10 orders of magnitude), and tremendous heterogeneity (due to post-translational …

Matrix-assisted laser desorption/ionization (MALDI) coupled with ion mobility–mass
spectrometry (IM–MS) provides a rapid (μs–ms) means for the two-dimensional (2D) …

Chemical cross‐linking of proteins, an established method in protein chemistry, has gained
renewed interest in combination with mass spectrometric analysis of the reaction products …

We describe and demonstrate a global strategy that extends the sensitivity, dynamic range,
comprehensiveness, and throughput of proteomic measurements based upon the use of …