Enzymes can be engineered at the level of their amino acid sequences to optimize key
properties such as expression, stability, substrate range, and catalytic efficiency─ or even to …

Recent progress in engineering highly promising biocatalysts has increasingly involved
machine learning methods. These methods leverage existing experimental and simulation …

Catalysis has a central role in organic synthetic methodology, especially in stereoselective
reactions. In many reactions, enantioselectivity is made possible through the use of chiral …

Ketones are crucial intermediates in synthesis and frequent moieties in many products. The
direct regioselective synthesis of ketones from internal alkenes could simplify synthetic …

In recent years, the engineering of flexible loops to improve enzyme properties has gained
attention in biocatalysis. Herein, we report a loop engineering strategy to improve the …

Steric hindrance modification-based pocket resha** is an effective approach for enzyme–
substrate acceptance evolution. However, previous studies were limited to using the …

This review analyzes a development in biochemistry, enzymology and biotechnology that
originally came as a surprise. Following the establishment of directed evolution of …

Cytochrome P450 BM3 monooxygenase is the topic of extensive research as many
researchers have evolved this enzyme to generate a variety of products. However, the …

Directed evolution is a powerful tool for improving existing properties and imparting
completely new functionalities to proteins,,–. Nonetheless, its potential in even small …

The widespread trade-off between stability and activity severely limits enzyme evolution.
Although some progresses have been made to overcome this limitation, the counteraction …