Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive

D Hering, A Borja, JI Jones, D Pont, P Boets… - Water research, 2018 - Elsevier
Assessment of ecological status for the European Water Framework Directive (WFD) is
based on “Biological Quality Elements”(BQEs), namely phytoplankton, benthic flora, benthic …

Next‐generation sequencing technologies for environmental DNA research

S Shokralla, JL Spall, JF Gibson… - Molecular …, 2012 - Wiley Online Library
Since 2005, advances in next‐generation sequencing technologies have revolutionized
biological science. The analysis of environmental DNA through the use of specific gene …

Structural variability and niche differentiation in the rhizosphere and endosphere bacterial microbiome of field-grown poplar trees

B Beckers, M Op De Beeck, N Weyens, W Boerjan… - Microbiome, 2017 - Springer
Background The plant microbiome represents one of the key determinants of plant health
and productivity by providing a plethora of functional capacities such as access to low …

Sequence depth, not PCR replication, improves ecological inference from next generation DNA sequencing

DP Smith, KG Peay - PloS one, 2014 - journals.plos.org
Recent advances in molecular approaches and DNA sequencing have greatly progressed
the field of ecology and allowed for the study of complex communities in unprecedented …

Promises and pitfalls of using high‐throughput sequencing for diet analysis

A Alberdi, O Aizpurua, K Bohmann… - Molecular ecology …, 2019 - Wiley Online Library
The application of high‐throughput sequencing‐based approaches to DNA extracted from
environmental samples such as gut contents and faeces has become a popular tool for …

Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring

DW Yu, Y Ji, BC Emerson, X Wang, C Ye… - Methods in Ecology …, 2012 - Wiley Online Library
Traditional biodiversity assessment is costly in time, money and taxonomic expertise.
Moreover, data are frequently collected in ways (eg visual bird lists) that are unsuitable for …

Tree diversity and species identity effects on soil fungi, protists and animals are context dependent

L Tedersoo, M Bahram, T Cajthaml, S Põlme… - The ISME …, 2016 - academic.oup.com
Plant species richness and the presence of certain influential species (sampling effect) drive
the stability and functionality of ecosystems as well as primary production and biomass of …

Parasites dominate hyperdiverse soil protist communities in Neotropical rainforests

F Mahé, C de Vargas, D Bass, L Czech… - Nature ecology & …, 2017 - nature.com
High animal and plant richness in tropical rainforest communities has long intrigued
naturalists. It is unknown if similar hyperdiversity patterns are reflected at the microbial scale …

Applications of next-generation sequencing to phylogeography and phylogenetics

JE McCormack, SM Hird, AJ Zellmer… - Molecular phylogenetics …, 2013 - Elsevier
This is a time of unprecedented transition in DNA sequencing technologies. Next-generation
sequencing (NGS) clearly holds promise for fast and cost-effective generation of multilocus …

The role of ciliate protozoa in the rumen

CJ Newbold, G De La Fuente, A Belanche… - Frontiers in …, 2015 - frontiersin.org
First described in 1843, Rumen protozoa with their striking appearance were assumed to be
important for the welfare of their host. However, despite contributing up to 50% of the bio …