Nsp3 of coronaviruses: Structures and functions of a large multi-domain protein

J Lei, Y Kusov, R Hilgenfeld - Antiviral research, 2018 - Elsevier
The multi-domain non-structural protein 3 (Nsp3) is the largest protein encoded by the
coronavirus (CoV) genome, with an average molecular mass of about 200 kD. Nsp3 is an …

From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses

R Hilgenfeld, M Peiris - Antiviral research, 2013 - Elsevier
This article introduces a series of invited papers in Antiviral Research marking the 10th
anniversary of the outbreak of severe acute respiratory syndrome (SARS), caused by a …

Boron containing compounds as protease inhibitors

R Smoum, A Rubinstein, VM Dembitsky… - Chemical …, 2012 - ACS Publications
Proteases form one of the largest and more important groups of enzymes. They selectively
catalyze the hydrolysis of peptide bonds and can be divided into four major classes …

Crystal structures of SARS-CoV-2 ADP-ribose phosphatase: from the apo form to ligand complexes

K Michalska, Y Kim, R Jedrzejczak, NI Maltseva, L Stols… - IUCrJ, 2020 - journals.iucr.org
Among 15 nonstructural proteins (Nsps), the newly emerging Severe Acute Respiratory
Syndrome coronavirus 2 (SARS-CoV-2) encodes a large, multidomain Nsp3. One of its units …

Molecular basis for ADP-ribose binding to the Mac1 domain of SARS-CoV-2 nsp3

DN Frick, RS Virdi, N Vuksanovic, N Dahal… - Biochemistry, 2020 - ACS Publications
The virus that causes COVID-19, SARS-CoV-2, has a large RNA genome that encodes
numerous proteins that might be targets for antiviral drugs. Some of these proteins, such as …

Bioinformatics and functional analyses of coronavirus nonstructural proteins involved in the formation of replicative organelles

BW Neuman - Antiviral research, 2016 - Elsevier
Replication of eukaryotic positive-stranded RNA viruses is usually linked to the presence of
membrane-associated replicative organelles. The purpose of this review is to discuss the …

Macro domain from Middle East respiratory syndrome coronavirus (MERS-CoV) is an efficient ADP-ribose binding module: crystal structure and biochemical studies

CC Cho, MH Lin, CY Chuang, CH Hsu - Journal of Biological Chemistry, 2016 - ASBMB
The newly emerging Middle East respiratory syndrome coronavirus (MERS-CoV) encodes
the conserved macro domain within non-structural protein 3. However, the precise …

Atlas of coronavirus replicase structure

BW Neuman, P Chamberlain, F Bowden, J Joseph - Virus research, 2014 - Elsevier
The international response to SARS-CoV has produced an outstanding number of protein
structures in a very short time. This review summarizes the findings of functional and …

X-ray structural and functional studies of the three tandemly linked domains of non-structural protein 3 (nsp3) from murine hepatitis virus reveal conserved functions

Y Chen, SN Savinov, AM Mielech, T Cao… - Journal of Biological …, 2015 - ASBMB
Murine hepatitis virus (MHV) has long served as a model system for the study of
coronaviruses. Non-structural protein 3 (nsp3) is the largest nsp in the coronavirus genome …

Increasing the X-ray diffraction power of protein crystals by dehydration: the case of bovine serum albumin and a survey of literature data

IR Krauss, F Sica, CA Mattia, A Merlino - International journal of molecular …, 2012 - mdpi.com
Serum albumin is one of the most widely studied proteins. It is the most abundant protein in
plasma with a typical concentration of 5 g/100 mL and the principal transporter of fatty acids …