Applications of nanobodies

S Muyldermans - Annual review of animal biosciences, 2021 - annualreviews.org
Unique, functional, homodimeric heavy chain–only antibodies, devoid of light chains, are
circulating in the blood of Camelidae. These antibodies recognize their cognate antigen via …

Nanobody-based products as research and diagnostic tools

T De Meyer, S Muyldermans, A Depicker - Trends in biotechnology, 2014 - cell.com
Since the serendipitous discovery 20 years ago of bona fide camelid heavy-chain
antibodies, their single-domain antigen-binding fragments, known as VHHs or nanobodies …

Microbial gas fermentation technology for sustainable food protein production

C Woern, L Grossmann - Biotechnology Advances, 2023 - Elsevier
The development of novel, sustainable, and robust food production technologies represents
one of the major pillars to address the most significant challenges humanity is going to face …

Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-map** and native protein complex isolation

T Pleiner, M Bates, S Trakhanov, CT Lee, JE Schliep… - elife, 2015 - elifesciences.org
Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies
against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to …

Expression of single-domain antibody in different systems

Y Liu, H Huang - Applied microbiology and biotechnology, 2018 - Springer
Camelid single-domain antibodies (sdAbs, VHHs, or Nanobodies®) are types of antibody
fragments that are composed of the heavy-chain variable domain only. These VHHs …

Nanobodies as molecular imaging probes

S Barakat, M Berksöz, P Zahedimaram, S Piepoli… - Free Radical Biology …, 2022 - Elsevier
Camelidae derived single-domain antibodies (sdAbs), commonly known as nanobodies
(Nbs), are the smallest antibody fragments with full antigen-binding capacity. Owing to their …

Recent advances in the selection and identification of antigen-specific nanobodies

W Liu, H Song, Q Chen, J Yu, M **an, R Nian… - Molecular …, 2018 - Elsevier
Nanobodies represent the next-generation antibody-derived biologics with significant
advances over conventional antibodies. Several rapid and robust techniques for isolating …

Brain delivery of single-domain antibodies: A focus on VHH and VNAR

E Pothin, D Lesuisse, P Lafaye - Pharmaceutics, 2020 - mdpi.com
Passive immunotherapy, ie, treatment with therapeutic antibodies, has been increasingly
used over the last decade in several diseases such as cancers or inflammation. However …

Active targeting of cancer cells by nanobody decorated polypeptide micelle with bio-orthogonally conjugated drug

SA Costa, D Mozhdehi, MJ Dzuricky, FJ Isaacs… - Nano …, 2018 - ACS Publications
Polypeptides are promising carriers for chemotherapeutics: they have minimal toxicity, can
be recombinantly synthesized with precise control over molecular weight, and enhance drug …

[HTML][HTML] Antibody fragments and their purification by protein L affinity chromatography

G Rodrigo, M Gruvegård, JM Van Alstine - Antibodies, 2015 - mdpi.com
Antibodies and related proteins comprise one of the largest and fastest-growing classes of
protein pharmaceuticals. A majority of such molecules are monoclonal antibodies; however …