Highlights•One-carbon compounds can be made from CO 2 to provide renewable microbial
feedstocks.•Methanol and formate have key advantages over gaseous one-carbon …

Abstract One-carbon (C1) substrates are preferred feedstocks for the biomanufacturing
industry and have recently gained attention owing to their natural abundance, low …

Engineering a biotechnological microorganism for growth on one-carbon intermediates,
produced from the abiotic activation of CO2, is a key synthetic biology step towards the …

Methanol, being electron rich and derivable from methane or CO 2, is a potentially
renewable one-carbon (C1) feedstock for microorganisms. Although the ribulose …

Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …

Methanol is a liquid with high energy storage capacity that holds promise as an alternative
substrate to replace sugars in the biotechnology industry. It can be produced from CO2 or …

Amino acid fermentation is one of the major pillars of industrial biotechnology. The multi-
billion USD amino acid market is rising steadily and is diversifying. Metabolic engineering is …

Methanol represents an attractive substrate for biotechnological applications. Utilization of
reduced one-carbon compounds for growth is currently limited to methylotrophic organisms …

Methane is a promising feedstock with high abundance and low cost for the sustainable
production of biochemicals and biofuels. Methanotrophic bacteria are particularly interesting …

Methylotrophy is a field of study dealing with microorganisms capable of utilization of
compounds devoid of carbon–carbon bonds (C1 compounds). In this review, we highlight …