Protein identification and peptide sequencing by tandem mass spectrometry requires
knowledge of how peptides fragment in the gas phase, specifically which bonds are broken …

This tutorial presents the most common ion activation techniques employed in tandem mass
spectrometry. In‐source fragmentation and metastable ion decompositions, as well as the …

Untitled Page 1 Page 2 JUNE 1, 2004 / ANALYTICAL CHEMISTRY 197 A he timeline for
maturation of a new measurement approach is difficult to predict but often accelerates as more …

The origin of the extent of charging and the mechanism by which multiply charged ions are
formed in electrospray ionization have been hotly debated for over a decade. Many factors …

The unfolding enthalpy of the native state of ubiquitin in solution is 5 to 8 times that of its
gaseous ions, as determined by electron capture dissociation (ECD) mass spectrometry …

This article provides a review of recent studies of the properties of unsolvated (and partially
solvated) peptides and proteins. The methods used to produce vapor-phase peptide and …

The influence of acid− base interactions on the gas-phase dissociation of a series of
protonated peptides was investigated. Peptides containing both acidic residues [aspartic …

A kinetic model, based on the “mobile proton” model of peptide fragmentation, was
developed to quantitatively simulate the low-energy collision-induced dissociation (CID) …

In this review we highlight the general change in perception in the field of ambient mass
spectrometry that it is the immediacy of sample preparation that differentiates the ambient …

Subfemtomole peptide sequence analysis has been achieved using microcapillary HPLC
columns, with integrated nanoelectrospray emitters, coupled directly to a Fourier transform …