Of ticks, mice and men: understanding the dual-host lifestyle of Lyme disease spirochaetes

JD Radolf, MJ Caimano, B Stevenson… - Nature reviews …, 2012 - nature.com
In little more than 30 years, Lyme disease, which is caused by the spirochaete Borrelia
burgdorferi, has risen from relative obscurity to become a global public health problem and a …

Cryo-electron tomography: the challenge of doing structural biology in situ

V Lučić, A Rigort, W Baumeister - Journal of Cell Biology, 2013 - rupress.org
Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …

The bacterial flagellar motor and its structural diversity

T Minamino, K Imada - Trends in microbiology, 2015 - cell.com
The bacterial flagellum is a reversible rotary motor powered by an electrochemical-potential
difference of specific ions across the cytoplasmic membrane. The H+-driven motor of …

Visualization of the type III secretion sorting platform of Shigella flexneri

B Hu, DR Morado, W Margolin… - Proceedings of the …, 2015 - National Acad Sciences
Bacterial type III secretion machines are widely used to inject virulence proteins into
eukaryotic host cells. These secretion machines are evolutionarily related to bacterial …

Structural diversity of bacterial flagellar motors

S Chen, M Beeby, GE Murphy, JR Leadbetter… - The EMBO …, 2011 - embopress.org
The bacterial flagellum is one of nature's most amazing and well‐studied nanomachines. Its
cell‐wall‐anchored motor uses chemical energy to rotate a microns‐long filament and …

Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold

M Beeby, DA Ribardo, CA Brennan… - Proceedings of the …, 2016 - National Acad Sciences
Although it is known that diverse bacterial flagellar motors produce different torques, the
mechanism underlying torque variation is unknown. To understand this difference better, we …

Architecture of the major component of the type III secretion system export apparatus

P Abrusci, M Vergara-Irigaray, S Johnson… - Nature structural & …, 2013 - nature.com
Type III secretion systems (T3SSs) are bacterial membrane–embedded nanomachines
designed to export specifically targeted proteins from the bacterial cytoplasm. Secretion …

Molecular architecture of chemoreceptor arrays revealed by cryoelectron tomography of Escherichia coli minicells

J Liu, B Hu, DR Morado, S Jani… - Proceedings of the …, 2012 - National Acad Sciences
The chemoreceptors of Escherichia coli localize to the cell poles and form a highly ordered
array in concert with the CheA kinase and the CheW coupling factor. However, a high …

The unique paradigm of spirochete motility and chemotaxis

NW Charon, A Cockburn, C Li, J Liu… - Annual review of …, 2012 - annualreviews.org
Spirochete motility is enigmatic: It differs from the motility of most other bacteria in that the
entire bacterium is involved in translocation in the absence of external appendages. Using …

Cellular electron cryotomography: toward structural biology in situ

CM Oikonomou, GJ Jensen - Annual review of biochemistry, 2017 - annualreviews.org
Electron cryotomography (ECT) provides three-dimensional views of macromolecular
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …