Label‐free molecular imaging of biological cells and tissues by linear and nonlinear Raman spectroscopic approaches

C Krafft, M Schmitt, IW Schie… - Angewandte Chemie …, 2017 - Wiley Online Library
Raman spectroscopy is an emerging technique in bioanalysis and imaging of biomaterials
owing to its unique capability of generating spectroscopic fingerprints. Imaging cells and …

The many facets of Raman spectroscopy for biomedical analysis

C Krafft, J Popp - Analytical and bioanalytical chemistry, 2015 - Springer
A critical review is presented on the use of linear and nonlinear Raman microspectroscopy
in biomedical diagnostics of bacteria, cells, and tissues. This contribution is combined with …

Raman spectroscopy and imaging in bioanalytics

D Cialla-May, C Krafft, P Rösch… - Analytical …, 2021 - ACS Publications
Since the discovery of the inelastic scattering of light, ie, the so-called Raman effect, 1
Raman spectroscopy has become an attractive tool in a high number of research fields …

Molecular imaging of live cells by Raman microscopy

AF Palonpon, M Sodeoka, K Fujita - Current opinion in chemical biology, 2013 - Elsevier
Highlights•Parallel detection of Raman spectra enables Raman imaging of live cells with
high spatial and temporal resolution.•Raman imaging of unstained live cells using 532 nm …

Redox imbalance and biochemical changes in cancer by probing redox-sensitive mitochondrial cytochromes in label-free visible resonance Raman imaging

H Abramczyk, B Brozek-Pluska, M Kopec, J Surmacki… - Cancers, 2021 - mdpi.com
Simple Summary Gliomas comprise around 30% of human brain tumors, while invasive
ductal carcinoma (IDC) comprises around 80% of human breast cancers. The aim of our …

Molecular component distribution imaging of living cells by multivariate curve resolution analysis of space-resolved Raman spectra

M Ando, H Hamaguchi - Journal of biomedical optics, 2014 - spiedigitallibrary.org
Label-free Raman microspectroscopy combined with a multivariate curve resolution (MCR)
analysis can be a powerful tool for studying a wide range of biomedical molecular systems …

Across the spectrum: integrating multidimensional metal analytics for in situ metallomic imaging

TJ Stewart - Metallomics, 2019 - academic.oup.com
To know how much of a metal species is in a particular location within a biological context at
any given time is essential for understanding the intricate roles of metals in biology and is …

A sensitive and specific Raman probe based on bisarylbutadiyne for live cell imaging of mitochondria

H Yamakoshi, A Palonpon, K Dodo, J Ando… - Bioorganic & medicinal …, 2015 - Elsevier
We previously showed that bisarylbutadiyne (BADY), which has a conjugated diyne
structure, exhibits an intense peak in the cellular Raman-silent region. Here, we synthesized …

Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

T Morimoto, H Kanda, H Kawagoe, T Ozawa… - Analyst, 2019 - pubs.rsc.org
Mitochondrial activity is a widely used criterion to judge the metabolic condition of a living
specimen. Numerous methods have been developed for related analyses, including the …

Raman spectroscopy provides a rapid, non‐invasive method for quantitation of starch in live, unicellular microalgae

Y Ji, Y He, Y Cui, T Wang, Y Wang, Y Li, WE Huang… - 2014 - Wiley Online Library
Conventional methods for quantitation of starch content in cells generally involve starch
extraction steps and are usually labor intensive, thus a rapid and non‐invasive method will …