Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d6/pyridine-d5

H Kim, J Ralph - Organic & biomolecular chemistry, 2010 - pubs.rsc.org
NMR fingerprinting of the components of finely divided plant cell walls swelled in DMSO has
been recently described. Cell wall gels, produced directly in the NMR tube with perdeutero …

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant

ND Bonawitz, JI Kim, Y Tobimatsu, PN Ciesielski… - Nature, 2014 - nature.com
Lignin is a phenylpropanoid-derived heteropolymer important for the strength and rigidity of
the plant secondary cell wall,. Genetic disruption of lignin biosynthesis has been proposed …

Tricin, a flavonoid monomer in monocot lignification

W Lan, F Lu, M Regner, Y Zhu, J Rencoret… - Plant …, 2015 - academic.oup.com
Tricin was recently discovered in lignin preparations from wheat (Triticum aestivum) straw
and subsequently in all monocot samples examined. To provide proof that tricin is involved …

Pathway of p-coumaric acid incorporation into maize lignin as revealed by NMR

J Ralph, RD Hatfield, S Quideau, RF Helm… - Journal of the …, 1994 - ACS Publications
NMR methods can be used to delineate detailed structural and regiochemical information on
the plant cell wall and elucidate biochemical incorporation pathways. Maize lignin isolated …

A facile spectroscopic method for measuring lignin content in lignocellulosic biomass

F Lu, C Wang, M Chen, F Yue, J Ralph - Green Chemistry, 2021 - pubs.rsc.org
Although measuring lignin contents is a routine operation for biomass compositional
analysis in process development aiming at efficient utilization of woody biomass, it is still a …

Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d 6

H Kim, J Ralph, T Akiyama - BioEnergy Research, 2008 - Springer
Although finely divided ball-milled whole cell walls do not completely dissolve in
dimethylsulfoxide (DMSO), they readily swell producing a gel. Solution-state two …

Quantitative 2D HSQC (Q-HSQC) via Suppression of J-Dependence of Polarization Transfer in NMR Spectroscopy:  Application to Wood Lignin

S Heikkinen, MM Toikka, PT Karhunen… - Journal of the …, 2003 - ACS Publications
A quantitative method to record 1H-13C correlation NMR spectra (Q-HSQC) is presented.
The suppression of 1 J CH-dependence is achieved by modulating the polarization transfer …

Abnormal lignin in a loblolly pine mutant

J Ralph, JJ MacKay, RD Hatfield, DM O'Malley… - Science, 1997 - science.org
Novel lignin is formed in a mutant loblolly pine (Pinus taeda L.) severely depleted in
cinnamyl alcohol dehydrogenase (EC 1.1. 1.195), which converts coniferaldehyde to …

Metabolic engineering of Escherichia coli for microbial synthesis of monolignols

Z Chen, X Sun, Y Li, Y Yan, Q Yuan - Metabolic engineering, 2017 - Elsevier
Monolignols are important plant metabolites involved in lignin biosynthesis. Their derivatives
exhibit various physiological and pharmaceutical functions. Here, efficient enzymes were …

Ligninolysis by a purified lignin peroxidase

KE Hammel, KA Jensen Jr, MD Mozuch… - Journal of Biological …, 1993 - Elsevier
The lignin peroxidases (LiPs) of white-rot basidiomycetes are generally thought to catalyze
the oxidative cleavage of polymeric lignin in vivo. However, direct evidence for such a role …