Gene regulation requires the dynamic coordination of hundreds of regulatory factors at
precise genomic and RNA targets. Although many regulatory factors have specific affinity for …

Recent advances in next-generation DNA sequencing and proteomics provide an
unprecedented ability to survey mRNA and protein abundances. Such proteome-wide …

Transcription factors (TFs) that bind common DNA motifs in vitro occupy distinct sets of
promoters in vivo, raising the question of how binding specificity is achieved. TFs are …

Transcriptomic profiling of complex tissues by single-nucleus RNA-sequencing (snRNA-seq)
affords some advantages over single-cell RNA-sequencing (scRNA-seq). snRNA-seq …

Quantitative views of cellular functions require precise measures of rates of biomolecule
production, especially proteins—the direct effectors of biological processes. Here, we …

Single molecule FISH (smFISH) allows studying transcription and RNA localization by
imaging individual mRNAs in single cells. We present smiFISH (single molecule …

The unabated progress in next-generation sequencing technologies is fostering a wave of
new genomics, epigenomics, transcriptomics and proteomics technologies. These …

Recent molecular studies have shown that, even when derived from a seemingly
homogenous population, individual cells can exhibit substantial differences in gene …

Promoter design remains one of the most important considerations in metabolic engineering
and synthetic biology applications. Theoretically, there are 450 possible sequences for a 50 …

Kindred cells can have different genomes because of dynamic changes in DNA. Single-cell
sequencing is needed to characterize these genomic differences but has been hindered by …