Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …

Caulobacter crescentus is an aquatic Gram-negative alphaproteobacterium that undergoes
multiple changes in cell shape, organelle production, subcellular distribution of proteins, and …

Mass-spectrometry-based methods for relative proteome quantification have broadly
affected life science research. However, important research directions, particularly those …

Chemoreceptor arrays are supramolecular transmembrane machines of unknown structure
that allow bacteria to sense their surroundings and respond by chemotaxis. We have …

Chemoreceptors are key components of the high-performance signal transduction system
that controls bacterial chemotaxis. Chemoreceptors are typically localized in a cluster at the …

Filament-forming cytoskeletal proteins are essential for the structure and organization of all
cells. Bacterial homologues of the major eukaryotic cytoskeletal families have now been …

The widely conserved ParABS system plays a major role in bacterial chromosome
segregation. How the components of this system work together to generate translocation …

The Escherichia coli chemotaxis network is a model system for biological signal processing.
In E. coli, transmembrane receptors responsible for signal transduction assemble into large …

The use of an alkane mixture that remains liquid at 77 K to freeze specimens has
advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of …

Electron cryotomography (ECT) provides three-dimensional views of macromolecular
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …