Analysing microbial community composition through amplicon sequencing: from sampling to hypothesis testing

LW Hugerth, AF Andersson - Frontiers in microbiology, 2017 - frontiersin.org
Microbial ecology as a scientific field is fundamentally driven by technological advance. The
past decade's revolution in DNA sequencing cost and throughput has made it possible for …

Metagenomics: tools and insights for analyzing next-generation sequencing data derived from biodiversity studies

A Oulas, C Pavloudi, P Polymenakou… - … and biology insights, 2015 - journals.sagepub.com
Advances in next-generation sequencing (NGS) have allowed significant breakthroughs in
microbial ecology studies. This has led to the rapid expansion of research in the field and …

Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2's q2-feature-classifier plugin

NA Bokulich, BD Kaehler, JR Rideout, M Dillon… - Microbiome, 2018 - Springer
Background Taxonomic classification of marker-gene sequences is an important step in
microbiome analysis. Results We present q2-feature-classifier (https://github. com/qiime2/q2 …

Important contribution of macroalgae to oceanic carbon sequestration

A Ortega, NR Geraldi, I Alam, AA Kamau… - Nature …, 2019 - nature.com
The role of macroalgae in Blue Carbon assessments has been controversial, partially due to
uncertainties about the fate of exported macroalgae. Available evidence suggests that …

Unifying the global phylogeny and environmental distribution of ammonia-oxidising archaea based on amoA genes

RJE Alves, BQ Minh, T Urich, A von Haeseler… - Nature …, 2018 - nature.com
Ammonia-oxidising archaea (AOA) are ubiquitous and abundant in nature and play a major
role in nitrogen cycling. AOA have been studied intensively based on the amoA gene …

Major role of nitrite-oxidizing bacteria in dark ocean carbon fixation

MG Pachiadaki, E Sintes, K Bergauer, JM Brown… - Science, 2017 - science.org
Carbon fixation by chemoautotrophic microorganisms in the dark ocean has a major impact
on global carbon cycling and ecological relationships in the ocean's interior, but the relevant …

Charting the complexity of the marine microbiome through single-cell genomics

MG Pachiadaki, JM Brown, J Brown, O Bezuidt… - Cell, 2019 - cell.com
Marine bacteria and archaea play key roles in global biogeochemistry. To improve our
understanding of this complex microbiome, we employed single-cell genomics and a …

Microbial community composition and diversity via 16S rRNA gene amplicons: evaluating the illumina platform

L Sinclair, OA Osman, S Bertilsson, A Eiler - PloS one, 2015 - journals.plos.org
As new sequencing technologies become cheaper and older ones disappear, laboratories
switch vendors and platforms. Validating the new setups is a crucial part of conducting …

Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi

CL Schoch, B Robbertse, V Robert, D Vu… - Database, 2014 - academic.oup.com
DNA phylogenetic comparisons have shown that morphology-based species recognition
often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data …

Bacterial diversity along a 2600 km river continuum

D Savio, L Sinclair, UZ Ijaz, J Parajka… - Environmental …, 2015 - Wiley Online Library
The bacterioplankton diversity in large rivers has thus far been under‐sampled despite the
importance of streams and rivers as components of continental landscapes. Here, we …