Regulation of long non-coding RNAs and genome dynamics by the RNA surveillance machinery

L Nair, H Chung, U Basu - Nature reviews Molecular cell biology, 2020 - nature.com
Much of the mammalian genome is transcribed, generating long non-coding RNAs
(lncRNAs) that can undergo post-transcriptional surveillance whereby only a subset of the …

RNA biogenesis and RNA metabolism factors as R-loop suppressors: a hidden role in genome integrity

R Luna, B Gómez-González… - Genes & …, 2024 - genesdev.cshlp.org
Genome integrity relies on the accuracy of DNA metabolism, but as appreciated for more
than four decades, transcription enhances mutation and recombination frequencies. More …

Noncoding RNA processing by DIS3 regulates chromosomal architecture and somatic hypermutation in B cells

B Laffleur, J Lim, W Zhang, Y Chen, E Pefanis… - Nature …, 2021 - nature.com
Noncoding RNAs are exquisitely titrated by the cellular RNA surveillance machinery for
regulating diverse biological processes. The RNA exosome, the predominant 3′ RNA …

[HTML][HTML] Helicase-dependent RNA decay illuminated by a cryo-EM structure of a human nuclear RNA exosome-MTR4 complex

EM Weick, MR Puno, K Januszyk, JC Zinder… - Cell, 2018 - cell.com
The ribonucleolytic RNA exosome interacts with RNA helicases to degrade RNA. To
understand how the 3′ to 5′ Mtr4 helicase engages RNA and the nuclear exosome, we …

Mechanism of noncoding RNA-associated N6-methyladenosine recognition by an RNA processing complex during IgH DNA recombination

L Nair, W Zhang, B Laffleur, MK Jha, J Lim, H Lee… - Molecular cell, 2021 - cell.com
Immunoglobulin heavy chain (IgH) locus-associated G-rich long noncoding RNA (SμGLT) is
important for physiological and pathological B cell DNA recombination. We demonstrate that …

[HTML][HTML] Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage

W Zhang, Z Yang, W Wang, Q Sun - nature communications, 2024 - nature.com
Abstract Transcription-replication conflicts (TRCs), especially Head-On TRCs (HO-TRCs)
can introduce R-loops and DNA damage, however, the underlying mechanisms are still …

EXOSC10 is required for RPA assembly and controlled DNA end resection at DNA double-strand breaks

J Domingo-Prim, M Endara-Coll, F Bonath… - Nature …, 2019 - nature.com
The exosome is a ribonucleolytic complex that plays important roles in RNA metabolism.
Here we show that the exosome is necessary for the repair of DNA double-strand breaks …

Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex

MR Puno, CD Lima - Cell, 2022 - cell.com
RNA quality control relies on co-factors and adaptors to identify and prepare substrates for
degradation by ribonucleases such as the 3′ to 5′ ribonucleolytic RNA exosome. Here …

RNA exosome drives early B cell development via noncoding RNA processing mechanisms

B Laffleur, CR Batista, W Zhang, J Lim, B Yang… - Science …, 2022 - science.org
B cell development is linked to successful V (D) J recombination, allowing B cell receptor
expression and ultimately antibody secretion for adaptive immunity. Germline noncoding …

[HTML][HTML] Helicases in R-loop formation and resolution

S Yang, L Winstone, S Mondal, Y Wu - Journal of Biological Chemistry, 2023 - Elsevier
With the development and wide usage of CRISPR technology, the presence of R-loop
structures, which consist of an RNA: DNA hybrid and a displaced single strand (ss) DNA …