Imagining the future of optical microscopy: everything, everywhere, all at once

H Balasubramanian, CM Hobson, TL Chew… - Communications …, 2023‏ - nature.com
The optical microscope has revolutionized biology since at least the 17th Century. Since
then, it has progressed from a largely observational tool to a powerful bioanalytical platform …

More than double the fun with two-photon excitation microscopy

P Luu, SE Fraser, F Schneider - Communications biology, 2024‏ - nature.com
For generations researchers have been observing the dynamic processes of life through the
lens of a microscope. This has offered tremendous insights into biological phenomena that …

Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and sha**

Z Qin, Z She, C Chen, W Wu, JKY Lau, NY Ip… - Nature …, 2022‏ - nature.com
High-resolution optical imaging deep in tissues is challenging because of optical
aberrations and scattering of light caused by the complex structure of living matter. Here we …

Adaptive optics for optical microscopy

Q Zhang, QI Hu, C Berlage, P Kner… - Biomedical Optics …, 2023‏ - opg.optica.org
Optical microscopy is widely used to visualize fine structures. When applied to bioimaging,
its performance is often degraded by sample-induced aberrations. In recent years, adaptive …

Speed scaling in multiphoton fluorescence microscopy

J Wu, N Ji, KK Tsia - Nature Photonics, 2021‏ - nature.com
After over 30 years of advances, multiphoton microscopy (MPM) is now instrumental in a
wide range of in vivo biological imaging applications. However, it has, until recently …

Astrocyte-secreted IL-33 mediates homeostatic synaptic plasticity in the adult hippocampus

Y Wang, WY Fu, K Cheung, KW Hung, C Chen… - Proceedings of the …, 2021‏ - pnas.org
Hippocampal synaptic plasticity is important for learning and memory formation.
Homeostatic synaptic plasticity is a specific form of synaptic plasticity that is induced upon …

Second Near‐Infrared (NIR‐II) Window for Imaging‐Navigated Modulation of Brain Structure and Function

K Zhang, FR Chen, L Wang, J Hu - Small, 2023‏ - Wiley Online Library
For a long time, optical imaging of the deep brain with high resolution has been a challenge.
Recently, with the advance in second near‐infrared (NIR‐II) bioimaging techniques and …

Construction and use of an adaptive optics two-photon microscope with direct wavefront sensing

P Yao, R Liu, T Broggini, M Thunemann, D Kleinfeld - Nature protocols, 2023‏ - nature.com
Two-photon microscopy, combined with the appropriate optical labelling, enables the
measurement and tracking of submicrometer structures within brain cells, as well as the …

Miniature two-photon microscopic imaging using dielectric metalens

C Wang, Q Chen, H Liu, R Wu, X Jiang, Q Fu… - Nano Letters, 2023‏ - ACS Publications
Miniature two-photon microscopy has emerged as a powerful technique for investigating
brain activity in freely moving animals. Ongoing research objectives include reducing probe …

Label-free metabolic and structural profiling of dynamic biological samples using multimodal optical microscopy with sensorless adaptive optics

RR Iyer, JE Sorrells, L Yang, EJ Chaney… - Scientific reports, 2022‏ - nature.com
Label-free optical microscopy has matured as a noninvasive tool for biological imaging; yet,
it is criticized for its lack of specificity, slow acquisition and processing times, and weak and …