Efforts to determine the antibody repertoire encoded by B cells in the blood or lymphoid
organs using high-throughput DNA sequencing technologies have been advancing at an …

The intestinal mucosa contains the largest population of antibody-secreting plasma cells in
the body, and in humans several grams of secretory immunoglobulin A (SIgA) are released …

Single-cell RNA sequencing has recently emerged as a powerful tool for map** cellular
heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for …

We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

This review summarizes recent developments in microfluidic platform approaches. In
contrast to isolated application-specific solutions, a microfluidic platform provides a set of …

B cell and T cell receptor repertoires compose the adaptive immune receptor repertoire
(AIRR) of an individual. The AIRR is a unique collection of antigen-specific receptors that …

Many of life's most fascinating phenomena emerge from interactions among many elements—
many amino acids determine the structure of a single protein, many genes determine the …

Antibody-antigen binding relies on the specific interaction of amino acids at the paratope-
epitope interface. The predictability of antibody-antigen binding is a prerequisite for de novo …

Each B-cell receptor consists of a pair of heavy and light chains. High-throughput
sequencing can identify large numbers of heavy-and light-chain variable regions (VH and …

Motivation High-throughput sequencing of large immune repertoires has enabled the
development of methods to predict the probability of generation by V (D) J recombination of …