Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2021 - Wiley Online Library
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when
performing flow cytometry experiments and includes comprehensive sections describing …

Phenotypical characterization of regulatory T cells in humans and rodents

AL Rodríguez-Perea, ED Arcia… - Clinical & …, 2016 - academic.oup.com
Regulatory T cells (Tregs) constitute a fascinating subpopulation of CD4+ T cells due to their
ability to limit the immune response against self and non-self antigens. Murine models and …

RNA-Seq signatures normalized by mRNA abundance allow absolute deconvolution of human immune cell types

G Monaco, B Lee, W Xu, S Mustafah, YY Hwang… - Cell reports, 2019 - cell.com
The molecular characterization of immune subsets is important for designing effective
strategies to understand and treat diseases. We characterized 29 immune cell types within …

OMIP‐069: forty‐color full spectrum flow cytometry panel for deep immunophenoty** of major cell subsets in human peripheral blood

LM Park, J Lannigan, MC Jaimes - Cytometry Part A, 2020 - Wiley Online Library
PURPOSE AND APPROPRIATE SAMPLE TYPES This 40‐color flow cytometry‐based panel
was developed for in‐depth immunophenoty** of the major cell subsets present in human …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

The genetic architecture of the human immune system: a bioresource for autoimmunity and disease pathogenesis

M Roederer, L Quaye, M Mangino, MH Beddall… - Cell, 2015 - cell.com
Despite recent discoveries of genetic variants associated with autoimmunity and infection,
genetic control of the human immune system during homeostasis is poorly understood. We …

Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design

R Nguyen, S Perfetto, YD Mahnke… - Cytometry Part …, 2013 - Wiley Online Library
After compensation, the measurement errors arising from multiple fluorescences spilling into
each detector become evident by the spreading of nominally negative distributions …

T cell phenotype and T cell receptor repertoire in patients with major depressive disorder

K Patas, A Willing, C Demiralay, JB Engler… - Frontiers in …, 2018 - frontiersin.org
While a link between inflammation and the development of neuropsychiatric disorders,
including major depressive disorder (MDD) is supported by a growing body of evidence …

OMIP‐94: twenty‐four‐color (Thirty‐marker) panel for deep immunophenoty** of immune cells in human peripheral blood

A Pierzchalski, AC Zenclussen, G Herberth - Cytometry Part A, 2023 - Wiley Online Library
Abstract This newly established 24‐color (30‐marker) panel focuses on the characterization
of the main human immune cell subtypes and was optimized for the analysis of human …

OMIP-042: 21-color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral blood

KW Staser, W Eades, J Choi… - Cytometry. Part A …, 2017 - pmc.ncbi.nlm.nih.gov
Background We initially designed this panel for the analysis of the PBMCs from patients who
have undergone alloHSCT, particularly those enrolled in drug studies for the prevention and …