Laboratory water-window x-ray microscopy
Soft x-ray microscopy in the water window (∼ 285− 535 e V) is an emerging and unique tool
for 2D and 3D imaging of unstained intact cellular samples in their near-native state with few …
for 2D and 3D imaging of unstained intact cellular samples in their near-native state with few …
Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells
Cryo-soft X-ray tomography is an imaging technique that addresses the need for mesoscale
imaging of cellular ultrastructure of relatively thick samples without the need for staining or …
imaging of cellular ultrastructure of relatively thick samples without the need for staining or …
Spontaneous driving forces give rise to protein− RNA condensates with coexisting phases and complex material properties
Phase separation of multivalent protein and RNA molecules underlies the biogenesis of
biomolecular condensates such as membraneless organelles. In vivo, these condensates …
biomolecular condensates such as membraneless organelles. In vivo, these condensates …
Nuclear and cytoplasmic spatial protein quality control is coordinated by nuclear–vacuolar junctions and perinuclear ESCRT
Effective protein quality control (PQC), essential for cellular health, relies on spatial
sequestration of misfolded proteins into defined inclusions. Here we reveal the coordination …
sequestration of misfolded proteins into defined inclusions. Here we reveal the coordination …
Nitrogen-fixing organelle in a marine alga
Symbiotic interactions were key to the evolution of chloroplast and mitochondria organelles,
which mediate carbon and energy metabolism in eukaryotes. Biological nitrogen fixation, the …
which mediate carbon and energy metabolism in eukaryotes. Biological nitrogen fixation, the …
Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells
High-resolution and rapid imaging of host cell ultrastructure can generate insights toward
viral disease mechanism, for example for a severe acute respiratory syndrome coronavirus …
viral disease mechanism, for example for a severe acute respiratory syndrome coronavirus …
[HTML][HTML] Soft X‐ray tomograms provide a structural basis for whole‐cell modeling
Develo** in silico models that accurately reflect a whole, functional cell is an ongoing
challenge in biology. Current efforts bring together mathematical models, probabilistic …
challenge in biology. Current efforts bring together mathematical models, probabilistic …
Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures
Abstract 3D correlative microscopy methods have revolutionized biomedical research,
allowing the acquisition of multidimensional information to gain an in-depth understanding …
allowing the acquisition of multidimensional information to gain an in-depth understanding …
Soft X-ray tomography to map and quantify organelle interactions at the mesoscale
Inter-organelle interactions are a vital part of normal cellular function; however, these have
proven difficult to quantify due to the range of scales encountered in cell biology and the …
proven difficult to quantify due to the range of scales encountered in cell biology and the …
Regulation of Oxygenic Photosynthesis during Trophic Transitions in the Green Alga Chromochloris zofingiensis
Light and nutrients are critical regulators of photosynthesis and metabolism in plants and
algae. Many algae have the metabolic flexibility to grow photoautotrophically …
algae. Many algae have the metabolic flexibility to grow photoautotrophically …