Protein-protein interactions are fundamental to the formation of intricate interaction networks
and the assembly of multisubunit protein complexes that represent the functional …

Formaldehyde has been used for decades to probe macromolecular structure and function
and to trap complexes, cells, and tissues for further analysis. Formaldehyde crosslinking is …

Transposable elements are viewed as 'selfish genetic elements', yet they contribute to gene
regulation and genome evolution in diverse ways. More than half of the human genome …

Despite the diverse biological pathways known to be regulated by ubiquitylation, global
identification of substrates that are targeted for ubiquitylation has remained a challenge. To …

Streptavidin and its homologs (together referred to as streptavidin) are widely used in
molecular science owing to their highly selective and stable interaction with biotin. Other …

The communication between cells and between cellular organelles is often controlled by the
interaction of membrane proteins. Although many methods for the detection of protein …

Transient interactions, which involve protein interactions that are formed and broken easily,
are important in many aspects of cellular function. Here we describe structural and functional …

The posttranslational addition of ubiquitin (Ub) helps control the half-life, localization, and
action of many intracellular plant proteins. A primary function is the degradation of …

The quiescent center (QC) plays an essential role during root development by creating a
microenvironment that preserves the stem cell fate of its surrounding cells. Despite being …

Protein modifications provide cells with exquisite temporal and spatial control of protein
function. Ubiquitin is among the most important modifiers, serving both to target hundreds of …